GSE8532-GSM211800-GPL5482-PMID:18391462.tsv
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"E. coli W3110 grown at LB-glucose medium (IPTG induction) in chemostat (D=0.1 h-1)" "characteristics_ch1.1"
"E. coli W3110/ppc grown at LB-glucose medium (IPTG induction) in chemostat (D=0.1 h-1)" "characteristics_ch2.1"
"Scanned images were analyzed with GenePix Pro 3.0 software (Axon Instruments, Union City, CA) to obtain gene expression ratios. Logged gene expression ratios were normalized by LOWESS regression (Yang et al., 2002) using the GeneSpring GX 7.3 software (Agilent Technologies)." "data_processing.1"
"Transcriptome of cells was prepared using a RNA extraction kit (RNeasy mini kit;Qiagen, Hilden, Germany)" "extract_protocol_ch1.1"
"Transcriptome of cells was prepared using a RNA extraction kit (RNeasy mini kit;Qiagen, Hilden, Germany)" "extract_protocol_ch2.1"
"The medium for continuous culture contained 20 g LB, 9 g glucose, 10 g NaHCO3, 50 mg ampicillin per liter and isopropyl--D-thiogalactopyranoside (IPTG) was supplemented to be 0.1 mM. The initial pH of the medium was set to be pH 7.3 by adding 1 N HCl solution. The inoculation culture was transferred into a 250 mL-fermentor containing 100 mL medium (Small scale multi-chemostat fermentor; Biotron Inc., Bucheon, Korea). The culture was performed at 37 C, 350 rpm with flushing CO2 gas at 20 mL/min. After 8 h inoculation, the feeding and outlet pumps were started at flow rate of 0.166 mL/h (dilution rate = 0.1 h-1). " "growth_protocol_ch1.1"
"The medium for continuous culture contained 20 g LB, 9 g glucose, 10 g NaHCO3, 50 mg ampicillin per liter and isopropyl--D-thiogalactopyranoside (IPTG) was supplemented to be 0.1 mM. The initial pH of the medium was set to be pH 7.3 by adding 1 N HCl solution. The inoculation culture was transferred into a 250 mL-fermentor containing 100 mL medium (Small scale multi-chemostat fermentor; Biotron Inc., Bucheon, Korea). The culture was performed at 37 C, 350 rpm with flushing CO2 gas at 20 mL/min. After 8 h inoculation, the feeding and outlet pumps were started at flow rate of 0.166 mL/h (dilution rate = 0.1 h-1). " "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"E. coli W3110 (KCTC 2223) at LB-GlC medium, D=0.1 h-1" "source_name_ch1.1"
"E. coli W3110 expressing ppc at LB-GlC medium, D=0.1 h-1" "source_name_ch2.1"
"PPC_LB-GlC medium_D=0.1 h-1" "title.1"
"E. coli W3110 grown at LB-glucose medium (IPTG induction) in chemostat (D=0.1 h-1)" "characteristics_ch1.1"
"E. coli W3110/ppc grown at LB-glucose medium (IPTG induction) in chemostat (D=0.1 h-1)" "characteristics_ch2.1"
"The medium for continuous culture contained 20 g LB, 9 g glucose, 10 g NaHCO3, 50 mg ampicillin per liter and isopropyl--D-thiogalactopyranoside (IPTG) was supplemented to be 0.1 mM. The initial pH of the medium was set to be pH 7.3 by adding 1 N HCl solution. The inoculation culture was transferred into a 250 mL-fermentor containing 100 mL medium (Small scale multi-chemostat fermentor; Biotron Inc., Bucheon, Korea). The culture was performed at 37 C, 350 rpm with flushing CO2 gas at 20 mL/min. After 8 h inoculation, the feeding and outlet pumps were started at flow rate of 0.166 mL/h (dilution rate = 0.1 h-1). " "growth_protocol_ch1.1"
"The medium for continuous culture contained 20 g LB, 9 g glucose, 10 g NaHCO3, 50 mg ampicillin per liter and isopropyl--D-thiogalactopyranoside (IPTG) was supplemented to be 0.1 mM. The initial pH of the medium was set to be pH 7.3 by adding 1 N HCl solution. The inoculation culture was transferred into a 250 mL-fermentor containing 100 mL medium (Small scale multi-chemostat fermentor; Biotron Inc., Bucheon, Korea). The culture was performed at 37 C, 350 rpm with flushing CO2 gas at 20 mL/min. After 8 h inoculation, the feeding and outlet pumps were started at flow rate of 0.166 mL/h (dilution rate = 0.1 h-1). " "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"E. coli W3110 (KCTC 2223) at LB-GlC medium, D=0.1 h-1" "source_name_ch1.1"
"E. coli W3110 expressing ppc at LB-GlC medium, D=0.1 h-1" "source_name_ch2.1"