GSE79305-GSM2091158-GPL13359-PMID:27252779.tsv
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"strain: JM109" "characteristics_ch1.1"
"genotype/variation: Wild type strain" "characteristics_ch1.2"
"phenotype: 0.6% n-butanol tolerance" "characteristics_ch1.3"
"Data was extracted with Agilent Feature Extraction software 10.7 (Agilent) using default parameters (protocol GE1-v1_91) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded." "data_processing.1"
"Total RNA was extracted using Qiagen RNeasy kit (Hilden, Germany) following manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNeasy mini kit (QIAGEN, GmBH, Germany) and RNase‐Free DNase Set (QIAGEN, GmBH, Germany)." "extract_protocol_ch1.1"
"E. coli strains harboring σ70 mutant B8 and WT were cultured overnight and inoculated (1%) into fresh medium." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Control, 0.8%butanol,1.5h,replicate 1" "source_name_ch1.1"
"Control109_0.8%Bu_1.5h_rep1" "title.1"
"n-Butanol (0.8%, v/v) was added at 0.8 OD660 and further incubated for 1.5 h." "treatment_protocol_ch1.1"
"strain: JM109" "characteristics_ch1.1"
"genotype/variation: Wild type strain" "characteristics_ch1.2"
"phenotype: 0.6% n-butanol tolerance" "characteristics_ch1.3"
"E. coli strains harboring σ70 mutant B8 and WT were cultured overnight and inoculated (1%) into fresh medium." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Control, 0.8%butanol,1.5h,replicate 1" "source_name_ch1.1"
"n-Butanol (0.8%, v/v) was added at 0.8 OD660 and further incubated for 1.5 h." "treatment_protocol_ch1.1"