GSE77177-GSM2045574-GPL21352-PMID:26872975.tsv
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"strain: MG1655" "characteristics_ch1.1"
"genotype: delta-rac" "characteristics_ch1.2"
"plasmid: G324D Rho mutant" "characteristics_ch1.3"
"Images Analysis using Agilent Feature Extraction Software Version 11.5 to obtain raw data.Probe intensities generated from feature extraction raw data were split based on channel type and analysis was performed." "data_processing.1"
"The RNA extraction from the samples was performed by Qiagen RNeasy Mini Kit with DNAse treatment (Cat.No. 74004) as per manufacturer’s protocol." "extract_protocol_ch1.1"
"Bacterial culture grown overnight at 37C, given 1% sub-culturing till OD at 600nm was 0.4" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Bacterial cell pellet" "source_name_ch1.1"
"E. coli MG1655 ∆Rac G324D, treated-Replicate2-mutant" "title.1"
"Treated experimental sets transformed with plasmids containing Rho (WT/N340S/G324D).Cells subjected to P1 transduction to delete the genomic counterparts of the gene." "treatment_protocol_ch1.1"
"strain: MG1655" "characteristics_ch1.1"
"genotype: delta-rac" "characteristics_ch1.2"
"plasmid: G324D Rho mutant" "characteristics_ch1.3"
"Bacterial culture grown overnight at 37C, given 1% sub-culturing till OD at 600nm was 0.4" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Bacterial cell pellet" "source_name_ch1.1"
"Treated experimental sets transformed with plasmids containing Rho (WT/N340S/G324D).Cells subjected to P1 transduction to delete the genomic counterparts of the gene." "treatment_protocol_ch1.1"