GSE77009-GSM2042402-GPL21351-PMID:26872975.tsv
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"strain: MG1655" "characteristics_ch1.1"
"plasmid: R258C NuSA" "characteristics_ch1.2"
"Images were quantified using Feature Extraction Software ( Agilent). Feature extracted raw data was analyzed using GeneSpring GX Version software from Agilent. Normalization of the data was done in GeneSpring GX using the 75th percentile shift" "data_processing.1"
"The RNA extraction from the samples was performed by Qiagen RNeasy Mini Kit with DNAse treatment (Cat.No. 74004) as per manufacturer’s protocol." "extract_protocol_ch1.1"
"Bacterial culture grown overnight at 37C, given 1% sub-culturing till OD at 600nm was 0.4" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Bacterial cell pellet" "source_name_ch1.1"
"MG1655 R258C NusA BR2, treated" "title.1"
"Treated experimental sets transformed with plasmids containing WT/G181D/R258C NusA. Cells subjected to P1 transduction to delete the genomic counterparts of the respective gene." "treatment_protocol_ch1.1"
"strain: MG1655" "characteristics_ch1.1"
"plasmid: R258C NuSA" "characteristics_ch1.2"
"Bacterial culture grown overnight at 37C, given 1% sub-culturing till OD at 600nm was 0.4" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Bacterial cell pellet" "source_name_ch1.1"
"Treated experimental sets transformed with plasmids containing WT/G181D/R258C NusA. Cells subjected to P1 transduction to delete the genomic counterparts of the respective gene." "treatment_protocol_ch1.1"