GSE77009-GSM2042400-GPL21351-PMID:26872975.tsv 1.43 KB
"strain: MG1655"	"characteristics_ch1.1"
"plasmid: G181D NusA"	"characteristics_ch1.2"
"Images were quantified using Feature Extraction Software ( Agilent). Feature extracted raw data was analyzed using GeneSpring GX Version  software from Agilent. Normalization of the data was done in GeneSpring GX using the 75th percentile shift"	"data_processing.1"
"The RNA extraction from the samples was performed by Qiagen RNeasy Mini Kit with DNAse treatment (Cat.No. 74004) as per manufacturer’s protocol."	"extract_protocol_ch1.1"
"Bacterial culture grown overnight at 37C, given 1% sub-culturing till OD at 600nm was 0.4"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Bacterial cell pellet"	"source_name_ch1.1"
"MG1655 G181D NusA BR2, treated"	"title.1"
"Treated experimental sets transformed with plasmids containing WT/G181D/R258C NusA. Cells subjected to P1 transduction to delete the genomic counterparts of the respective gene."	"treatment_protocol_ch1.1"
"strain: MG1655"	"characteristics_ch1.1"
"plasmid: G181D NusA"	"characteristics_ch1.2"
"Bacterial culture grown overnight at 37C, given 1% sub-culturing till OD at 600nm was 0.4"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Bacterial cell pellet"	"source_name_ch1.1"
"Treated experimental sets transformed with plasmids containing WT/G181D/R258C NusA. Cells subjected to P1 transduction to delete the genomic counterparts of the respective gene."	"treatment_protocol_ch1.1"