"growth conditions: Minimal Medium A  supplemented with casaminoacids (0,1%) and succinate (0,2%),  OD 0,2 at 40°C"	"characteristics_ch1.1"
"chip antibody: none"	"characteristics_ch1.2"
"Basecalls performed using CASAVA version 1.8.2"	"data_processing.1"
"ChIP-seq reads were aligned to the E. coli NC_000913 genome using BWA 0.6.2"	"data_processing.2"
"Genome_build: NC_000913"	"data_processing.3"
"Supplementary_files_format_and_content: Enrichement (IP/input) wig files were generated using homemade matlab code"	"data_processing.4"
"Bacteria were lyzed with lyzozyme and sonicated to shear DNA. TopoIV-DNA complexes were isolated with antibody ."	"extract_protocol_ch1.1"
"Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (FC-104-5001). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols."	"extract_protocol_ch1.2"
"E. coli were grown in LB or minimal medium supplemented with casaminoacids (0,1%) and succinate (0,1%) until OD 0,2-0,4"	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli MG1655 parE::flag"	"source_name_ch1.1"
"Input ParE-flag S40min"	"title.1"
"For ChIP-seq experiments E. coli culture were fixed with formaldehyde (1% final concentration); for NorfliP experiments TopoIV was crosslinked with Norfloxacin (2µM)"	"treatment_protocol_ch1.1"
"ChIP-Seq"	"library_strategy.1"
"growth conditions: Minimal Medium A  supplemented with casaminoacids (0,1%) and succinate (0,2%),  OD 0,2 at 40°C"	"characteristics_ch1.1"
"chip antibody: none"	"characteristics_ch1.2"
"E. coli were grown in LB or minimal medium supplemented with casaminoacids (0,1%) and succinate (0,1%) until OD 0,2-0,4"	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli MG1655 parE::flag"	"source_name_ch1.1"
"For ChIP-seq experiments E. coli culture were fixed with formaldehyde (1% final concentration); for NorfliP experiments TopoIV was crosslinked with Norfloxacin (2µM)"	"treatment_protocol_ch1.1"