GSE74810-GSM1934004-GPL15010-GPL20046-PMID:26673755.tsv
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"strain: MG1655" "characteristics_ch1.1"
"chip antibody: Monoclonal anti-FLAG antibody, Murine IgG" "characteristics_ch1.2"
"chip antibody manufacturer/lot#: Sigma-Aldrich, M8823-1ML, Lot # SLBD7244V" "characteristics_ch1.3"
"Library strategy: ChIP-exo" "data_processing.1"
"Peconic LLC performed data processing" "data_processing.2"
"ChIP-exo reads were aligned using BWA with default parameters" "data_processing.3"
"Peaks were called with GeneTrack 1.0.3 (s=5, e=10)" "data_processing.4"
"Peaks on opposing strands were paired with Genetrack (u=0, d=80, b=2, m=mode)" "data_processing.5"
"Singleton peaks (standard deviation =0) and peaks without matched peaks on the opposing strand where discarded" "data_processing.6"
"Genome_build: NC_000913.3" "data_processing.7"
"Genome_build: NC_016856.1" "data_processing.8"
"Supplementary_files_format_and_content: Bedgraphs files representing the extracted peak data" "data_processing.9"
"Supplementary_files_format_and_content: chromosome chromosomeStartPosition chromosomeStopPosition readCoverage(positive values on + strand, negative on - strand)" "data_processing.10"
"E. coli and S. Typhimurium cells (at mid-exponential growth phase) were fixed with formaldehyde (1% final concentration) for 20 min at 30°C with shaking. The crosslinking reaction was then quenched by the addition of glycine (0.33M final concentrationg) for 5 minutes at room temperature with gentle mixing. Cells where then lysed on ice with lysozyme and sonication." "extract_protocol_ch1.1"
"Samples were used for on-bead enzymatic reactions of the ChIP-exo procedure and Illumina Tru-seq sequencing libraries were construction as described in Rhee and Pugh, 2012" "extract_protocol_ch1.2"
"E. coli cells were grown in Kornberg medium (1.1% [wt/vol] K2HPO4, 0.85% [wt/vol] KH2PO4, 0.6% [wt/vol] yeast extract containing 0.5% [wt/vol] glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm" "growth_protocol_ch1.1"
"S. Typhimurium cells were grown in LB (supplemented with 10mM glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm" "growth_protocol_ch1.2"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"UvrY (Response regulator of the BarA/UvrY two-component system)" "source_name_ch1.1"
"UvrY - ChIP-exo" "title.1"
"OTHER" "library_strategy.1"
"strain: MG1655" "characteristics_ch1.1"
"chip antibody: Monoclonal anti-FLAG antibody, Murine IgG" "characteristics_ch1.2"
"chip antibody manufacturer/lot#: Sigma-Aldrich, M8823-1ML, Lot # SLBD7244V" "characteristics_ch1.3"
"E. coli cells were grown in Kornberg medium (1.1% [wt/vol] K2HPO4, 0.85% [wt/vol] KH2PO4, 0.6% [wt/vol] yeast extract containing 0.5% [wt/vol] glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm" "growth_protocol_ch1.1"
"S. Typhimurium cells were grown in LB (supplemented with 10mM glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm" "growth_protocol_ch1.2"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"UvrY (Response regulator of the BarA/UvrY two-component system)" "source_name_ch1.1"