GSE74810-GSM1934004-GPL15010-GPL20046-PMID:26673755.tsv 3.04 KB
"strain: MG1655"	"characteristics_ch1.1"
"chip antibody: Monoclonal anti-FLAG antibody, Murine  IgG"	"characteristics_ch1.2"
"chip antibody manufacturer/lot#: Sigma-Aldrich, M8823-1ML, Lot #  SLBD7244V"	"characteristics_ch1.3"
"Library strategy: ChIP-exo"	"data_processing.1"
"Peconic LLC performed data processing"	"data_processing.2"
"ChIP-exo reads were aligned using BWA with  default parameters"	"data_processing.3"
"Peaks were called with GeneTrack 1.0.3 (s=5, e=10)"	"data_processing.4"
"Peaks on opposing strands were paired with Genetrack (u=0, d=80, b=2, m=mode)"	"data_processing.5"
"Singleton peaks (standard deviation =0) and peaks without matched peaks on the opposing strand where discarded"	"data_processing.6"
"Genome_build: NC_000913.3"	"data_processing.7"
"Genome_build: NC_016856.1"	"data_processing.8"
"Supplementary_files_format_and_content: Bedgraphs files representing the extracted peak data"	"data_processing.9"
"Supplementary_files_format_and_content: chromosome chromosomeStartPosition chromosomeStopPosition readCoverage(positive values on + strand, negative on - strand)"	"data_processing.10"
"E. coli and S. Typhimurium cells (at mid-exponential growth phase) were fixed with formaldehyde (1% final concentration) for  20 min at 30°C with shaking. The crosslinking reaction was then quenched by the addition of glycine (0.33M final concentrationg) for 5 minutes at room temperature with gentle mixing.  Cells where then lysed on ice with lysozyme and sonication."	"extract_protocol_ch1.1"
"Samples were used for on-bead enzymatic reactions of the ChIP-exo procedure and Illumina Tru-seq sequencing libraries were construction as described in Rhee and Pugh, 2012"	"extract_protocol_ch1.2"
"E. coli cells were grown in Kornberg medium (1.1% [wt/vol] K2HPO4, 0.85% [wt/vol] KH2PO4, 0.6% [wt/vol] yeast extract containing 0.5% [wt/vol] glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm"	"growth_protocol_ch1.1"
"S. Typhimurium cells were grown in LB (supplemented with 10mM glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm"	"growth_protocol_ch1.2"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"UvrY (Response regulator of the BarA/UvrY two-component system)"	"source_name_ch1.1"
"UvrY - ChIP-exo"	"title.1"
"OTHER"	"library_strategy.1"
"strain: MG1655"	"characteristics_ch1.1"
"chip antibody: Monoclonal anti-FLAG antibody, Murine  IgG"	"characteristics_ch1.2"
"chip antibody manufacturer/lot#: Sigma-Aldrich, M8823-1ML, Lot #  SLBD7244V"	"characteristics_ch1.3"
"E. coli cells were grown in Kornberg medium (1.1% [wt/vol] K2HPO4, 0.85% [wt/vol] KH2PO4, 0.6% [wt/vol] yeast extract containing 0.5% [wt/vol] glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm"	"growth_protocol_ch1.1"
"S. Typhimurium cells were grown in LB (supplemented with 10mM glucose) to mid-exponential phase (OD600 = ~0.6) at 37C and shaking 250rpm"	"growth_protocol_ch1.2"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"UvrY (Response regulator of the BarA/UvrY two-component system)"	"source_name_ch1.1"