GSE73640-GSM1899945-GPL20978-PMID:27681369.tsv 1.8 KB
"strain: BL21(DE3)"	"characteristics_ch1.1"
"stress: control"	"characteristics_ch1.2"
"metabolic response: BL21(DE3) is very susceptible to heptanoic acid, because several acid resistance systems stay inactive under this stress condition"	"characteristics_ch1.3"
"The average fluorescence intensity for each spot was calculated and local background was subtracted. All data normalization and selection of fold-changed genes were performed using GeneSpringGX 7.3.1 (Agilent Technology, USA).  Normalization for Agilent one-color method was performed, which is Data transformation : Set measurements less than 5.0 to 5.0 and Per Chip :Normalize to 50th percentage."	"data_processing.1"
"Total RNA was extracted using TRIzol (Invitrogen)"	"extract_protocol_ch1.1"
"When E. coli cells were cultivated to the exponential growth phase (OD600 = 0.5) in Riesenberg medium, 3 or 10 mM heptanoic acid was treated to BL21(DE3) and K-12 MG1655, respectively. After 3 h incubation, the E. coli cells were harvested for transcriptome analysis"	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"BL21(DE3) without heptanoic acid"	"source_name_ch1.1"
"BL21(DE3) HPA 0mM"	"title.1"
"strain: BL21(DE3)"	"characteristics_ch1.1"
"stress: control"	"characteristics_ch1.2"
"metabolic response: BL21(DE3) is very susceptible to heptanoic acid, because several acid resistance systems stay inactive under this stress condition"	"characteristics_ch1.3"
"When E. coli cells were cultivated to the exponential growth phase (OD600 = 0.5) in Riesenberg medium, 3 or 10 mM heptanoic acid was treated to BL21(DE3) and K-12 MG1655, respectively. After 3 h incubation, the E. coli cells were harvested for transcriptome analysis"	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"BL21(DE3) without heptanoic acid"	"source_name_ch1.1"