GSE69830-GSM1709741-GPL20323-PMID:28348816.tsv 1.91 KB
"strain: CFT073"	"characteristics_ch1.1"
"genotype: deltahmp deltanrfA deltanorVW"	"characteristics_ch1.2"
"RNA-seq reads were aligned to the CFT073 genome using bowtie (version 0.12.8)"	"data_processing.1"
"TPM (transcripts per million) was calculated by RSEM(version 1.2.19)"	"data_processing.2"
"Genome_build: CFT073(AE014075.1)"	"data_processing.3"
"Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample; column1: transcript id; column2: TPM value"	"data_processing.4"
"Total Rna was extracted from cultures using Qiagen RNeasy Protect Bacteria Mini Kit. For rRNA depletion, samples were treated with the MICROBExpressTM Bacterial mRNA Enrichment Kit (Life Technologies) according to the manufacturer’s instructions.  Samples were cleaned with the Zymo RNA Clean and ConcentratorTM (Zymo Research) and then subjected to a second cycle of rRNA depletion.  RNA was recovered by ethanol precipitation."	"extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols"	"extract_protocol_ch1.2"
"Samples were grown anaerobically in MOPS minimal media supplemented with casamino acids using glucose as the carbon source. 20mM nitrate was added to (+NO) cultures for inducing nitrosative stress."	"growth_protocol_ch1.1"
"Escherichia coli CFT073"	"organism_ch1.1"
"uropathogenic Escherichia coli (UPEC) grown with nitrosative stress"	"source_name_ch1.1"
"(+NO)1"	"title.1"
"RNA-Seq"	"library_strategy.1"
"strain: CFT073"	"characteristics_ch1.1"
"genotype: deltahmp deltanrfA deltanorVW"	"characteristics_ch1.2"
"Samples were grown anaerobically in MOPS minimal media supplemented with casamino acids using glucose as the carbon source. 20mM nitrate was added to (+NO) cultures for inducing nitrosative stress."	"growth_protocol_ch1.1"
"Escherichia coli CFT073"	"organism_ch1.1"
"uropathogenic Escherichia coli (UPEC) grown with nitrosative stress"	"source_name_ch1.1"