"growth temperature: 37°C"	"characteristics_ch1.1"
"chip antibody: anti-Flag"	"characteristics_ch1.2"
"genotype/variation: AB1157 mukB-3xflag"	"characteristics_ch1.3"
"Illumina Casava v1.8 used for basecalling."	"data_processing.1"
"Reads were adapter removed and trimmed to 40Bp using trimmomatic 0.32 with parameters ILLUMINACLIP:/Trimmomatic-0.32/adapters/TruSeq3-PE-2.fa:2:30:10 HEADCROP:8 CROP:40 SLIDINGWINDOW:6:5 MINLEN:40"	"data_processing.2"
"Sequences were mapped to NC_000913.2 using bowtie2 v2.2.2 with parameters -N 0 --sensitive --minins 130 --maxins 780 -q --no-mixed --no-discordant  --no-unal"	"data_processing.3"
"MACS v2.0.10.20131216 was used for peak calling with parameters --llocal=10000 -g 4639675 --bw 300 -p 0.05 --slocal=1000 --keep-dup=20"	"data_processing.4"
"Genome_build: NC_000913.2"	"data_processing.5"
"Supplementary_files_format_and_content: MACS v2.0.10.20131216 peaks file"	"data_processing.6"
"Cells were resuspended in Lysis buffer (10mM tris, 20% sucrose, 50mM NaCl, 10mM EDTA, 10mg/ml lysosyme, 0.1mg/ml EDTA, 0.1mg/ml RNaseA) and subsequently incubated for 30min at 37C. Next, IP buffer was added (50mM Hepes, 150mM NaCl, 1mM EDTA, 1%triton, 0.1% sodium deoxycholate, 0.1% SDS). Cells have been sonicated, centrifugated, and the supernatent was used for the immunoprecipitation."	"extract_protocol_ch1.1"
"Sequencing libraries were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (#E7370, NEB) for samples 1 to 3 (37 C) and the WaferGen PrepX ILM ChiP-Seq Library Kit (#400075, WaferGen) on the Apollo 324 System for samples 4 to 10 (22 C)."	"extract_protocol_ch1.2"
"Cells were grown in LB at 22°C or 37°C, as indicated."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"E. coli"	"source_name_ch1.1"
"MukB 37°C"	"title.1"
"Crosslinking was performed using 1% of forlmaldehyde"	"treatment_protocol_ch1.1"
"ChIP-Seq"	"library_strategy.1"
"growth temperature: 37°C"	"characteristics_ch1.1"
"chip antibody: anti-Flag"	"characteristics_ch1.2"
"genotype/variation: AB1157 mukB-3xflag"	"characteristics_ch1.3"
"Cells were grown in LB at 22°C or 37°C, as indicated."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"E. coli"	"source_name_ch1.1"
"Crosslinking was performed using 1% of forlmaldehyde"	"treatment_protocol_ch1.1"