GSE6609-GSM153310-GPL4698-PMID:17891379.tsv
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"pool of aliquots from all samples" "characteristics_ch1.1"
"day: . strain: . oxygen: ." "characteristics_ch2.1"
"Feature pixel median minus background pixel mean;" "data_processing.1"
"ratio set to missing where SNR (feature mean - backround mean / background SD) less than 2 in both channels ;" "data_processing.2"
"normalization subtracting array-median log2 ratio." "data_processing.3"
"Cells were harvested on days 1 and 3 of glucose starvation, with samples taken from the" "extract_protocol_ch1.1"
"same cultures used for the viability assay. For each experimental condition, 15 aliquots of" "extract_protocol_ch1.2"
"1.8 ml (ca. 3E+9 cells) were pipetted into 2.0 ml tubes and immediately centrifuged at" "extract_protocol_ch1.3"
"16,000 g for 40 s at room temperature. The supernatant was discarded, and cell pellets" "extract_protocol_ch1.4"
"were immediately frozen in liquid nitrogen and stored at -80 deg. C. For RNA isolation," "extract_protocol_ch1.5"
"triplicate samples were prepared by combining cells from 5 tubes each. Each pellet was" "extract_protocol_ch1.6"
"re-suspended in 200 ul Trizol and combined for a total volume of 1 ml, then promptly" "extract_protocol_ch1.7"
"chilled in a dry ice/ethanol. bath. After homogenization with glass beads,. RNA was" "extract_protocol_ch1.8"
"isolated by chloroform extraction and precipitation by isopropanol. Precipitated RNA" "extract_protocol_ch1.9"
"was washed with 70 percent ethanol, resuspended in 30 ul water, and held at 4 deg. C for" "extract_protocol_ch1.10"
"further processing" "extract_protocol_ch1.11"
"Cells were harvested on days 1 and 3 of glucose starvation, with samples taken from the" "extract_protocol_ch2.1"
"same cultures used for the viability assay. For each experimental condition, 15 aliquots of" "extract_protocol_ch2.2"
"1.8 ml (ca. 3E+9 cells) were pipetted into 2.0 ml tubes and immediately centrifuged at" "extract_protocol_ch2.3"
"16,000 g for 40 s at room temperature. The supernatant was discarded, and cell pellets" "extract_protocol_ch2.4"
"were immediately frozen in liquid nitrogen and stored at -80 deg. C. For RNA isolation," "extract_protocol_ch2.5"
"triplicate samples were prepared by combining cells from 5 tubes each. Each pellet was" "extract_protocol_ch2.6"
"re-suspended in 200 ul Trizol and combined for a total volume of 1 ml, then promptly" "extract_protocol_ch2.7"
"chilled in a dry ice/ethanol. bath. After homogenization with glass beads,. RNA was" "extract_protocol_ch2.8"
"isolated by chloroform extraction and precipitation by isopropanol. Precipitated RNA" "extract_protocol_ch2.9"
"was washed with 70 percent ethanol, resuspended in 30 ul water, and held at 4 deg. C for" "extract_protocol_ch2.10"
"further processing" "extract_protocol_ch2.11"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Reference Pool" "source_name_ch1.1"
"D3-AN18" "source_name_ch2.1"
"68923" "title.1"
"pool of aliquots from all samples" "characteristics_ch1.1"
"day: . strain: . oxygen: ." "characteristics_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Reference Pool" "source_name_ch1.1"
"D3-AN18" "source_name_ch2.1"