GSE64847-GSM1581563-GPL16085-PMID:29771928.tsv 2.83 KB
"strain: wt"	"characteristics_ch1.1"
"chip antibody: E. coli CRP Monoclonal Antibody, Neoclone, #N0004"	"characteristics_ch1.2"
"The MiSeq Reporter MiSeq Reporter 2.4.60.8 was used for basecalling and demultiplexing"	"data_processing.1"
"bowtie2 v2.2.3 was used for alignment"	"data_processing.2"
"Peak-calls were done using GPS within the GEMS software package (v2.3) adapted for ChIP-exo data"	"data_processing.3"
"Genome_build: NC_000913.2"	"data_processing.4"
"Supplementary_files_format_and_content: Processed data is presented in gff files containing a pileup of 5' tags. GFF column headers \"Genbank fna ID, file name, left genomic position, right genomic position, value, strand, extra value, information\""	"data_processing.5"
"Cells were enyzmatically lysed in the presence of protease inhibitors prior to fragmentation using sonication. Protein/DNA complexes were recovered using an appropriate mouse monoclonal antibody followed by recovery using Pan Mouse IgG Dynabeads. While complexed on the magnetic bead, a series of enzymatic reactions were performed to 1) end repair, fragmented DNA, 2) ligate sequencing adpator 2, 3) nick repair, 4) lambda exonuclease treatment, 5) RecJ nuclease treatment. This was based on the method developed by Rhee et al. (doi:10.1016/j.cell.2011.11.013). IP DNA was then rleased from the complex and recovered."	"extract_protocol_ch1.1"
"Library construction was based on the method described by Rhee et al. (doi:10.1016/j.cell.2011.11.013)."	"extract_protocol_ch1.2"
"Cells were grown in shake flasks to mid-exponential phase under either aerobic or anaerobic conditions depending on the sample. M9 minimal media supplement with either glucose, fructose, or glycerol as the sole carbon source was used depending on the sample."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Chromosomal DNA"	"source_name_ch1.1"
"ChIPExo-Crp_wt_glycerol_NH4Cl_O2_3_anti-crp"	"title.1"
"Cells were crosslinked in 1% formadehyde for 25 min at room temperature followed by 5 minutes of quenching with glycine. Cells were washed 3X with ice cold TBS. Cell pellets were stored at -80 C."	"treatment_protocol_ch1.1"
"ChIP-Seq"	"library_strategy.1"
"strain: wt"	"characteristics_ch1.1"
"chip antibody: E. coli CRP Monoclonal Antibody, Neoclone, #N0004"	"characteristics_ch1.2"
"Cells were grown in shake flasks to mid-exponential phase under either aerobic or anaerobic conditions depending on the sample. M9 minimal media supplement with either glucose, fructose, or glycerol as the sole carbon source was used depending on the sample."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Chromosomal DNA"	"source_name_ch1.1"
"Cells were crosslinked in 1% formadehyde for 25 min at room temperature followed by 5 minutes of quenching with glycine. Cells were washed 3X with ice cold TBS. Cell pellets were stored at -80 C."	"treatment_protocol_ch1.1"