GSE61749-GSM1513325-GPL13336-PMID:26474851.tsv 1.98 KB
"strain: DH1ΔleuB::gfpuv5-kmr"	"characteristics_ch1.1"
"condition: exponential growth, 43.2ºC"	"characteristics_ch1.2"
"Microarray data were processed using custom scripts written in R based on the finite hybridisation (FH) model (Ono et al, 2008) and the thermodynamic model of non-specific binding (NSB) on short nucleotide microarrays (Furusawa et al, 2009)."	"data_processing.1"
"The normalized data can be found on the series record in the file \"GSE61749_Normalized_data.txt\". The data values presented in this file are log10 mRNA concentration (pM)."	"data_processing.2"
"Total RNAs were extracted using an RNeasy mini kit (Qiagen) in accordance with the manufacturer’s instructions."	"extract_protocol_ch1.1"
"Cells were grown in the minimal media, M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli 43B evolved strain, under 43ºC, rep 3"	"source_name_ch1.1"
"E. coli 43B evolved strain, evolutionary temperature, rep 3"	"title.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 7,000 × g for 3 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"
"strain: DH1ΔleuB::gfpuv5-kmr"	"characteristics_ch1.1"
"condition: exponential growth, 43.2ºC"	"characteristics_ch1.2"
"Cells were grown in the minimal media, M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli 43B evolved strain, under 43ºC, rep 3"	"source_name_ch1.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 7,000 × g for 3 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"