GSE61749-GSM1513323-GPL13336-PMID:26474851.tsv
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"strain: DH1ΔleuB::gfpuv5-kmr" "characteristics_ch1.1"
"condition: exponential growth, 43.2ºC" "characteristics_ch1.2"
"Microarray data were processed using custom scripts written in R based on the finite hybridisation (FH) model (Ono et al, 2008) and the thermodynamic model of non-specific binding (NSB) on short nucleotide microarrays (Furusawa et al, 2009)." "data_processing.1"
"The normalized data can be found on the series record in the file \"GSE61749_Normalized_data.txt\". The data values presented in this file are log10 mRNA concentration (pM)." "data_processing.2"
"Total RNAs were extracted using an RNeasy mini kit (Qiagen) in accordance with the manufacturer’s instructions." "extract_protocol_ch1.1"
"Cells were grown in the minimal media, M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E. coli 43B evolved strain, under 43ºC, rep 1" "source_name_ch1.1"
"E. coli 43B evolved strain, evolutionary temperature, rep 1" "title.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 7,000 × g for 3 min at 4°C, and the pelleted cells were stored at –80°C prior to use." "treatment_protocol_ch1.1"
"strain: DH1ΔleuB::gfpuv5-kmr" "characteristics_ch1.1"
"condition: exponential growth, 43.2ºC" "characteristics_ch1.2"
"Cells were grown in the minimal media, M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E. coli 43B evolved strain, under 43ºC, rep 1" "source_name_ch1.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 7,000 × g for 3 min at 4°C, and the pelleted cells were stored at –80°C prior to use." "treatment_protocol_ch1.1"