GSE59441-GSM1436938-GPL3154-PMID:25534751.tsv 1.29 KB
"genotype/variation: WT"	"characteristics_ch1.1"
"Data was analyzed in the Affymetrix GenomeChipOperating Software (GCOS)."	"data_processing.1"
"Cells were lysed using a bead beater (Biospec) and mRNA was isolated using a Qiagen RNeasy mini kit (Cat# 74104)."	"extract_protocol_ch1.1"
"Strains were grown in LB at 37°C with shaking at 250 rpm.  16 h overnight cultures were diluted to OD600 = 0.05 in HEPES-glucose (0.4%), grown to OD600 ~ 0.6, and exposed to 4.5% deoxycholate for 30 min."	"growth_protocol_ch1.1"
"Escherichia coli BW25113"	"organism_ch1.1"
"BW25113 with deoxycholate stress"	"source_name_ch1.1"
"BW25113 HEPES-glu 30 min 4.5% deoxycholate"	"title.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice."	"treatment_protocol_ch1.1"
"genotype/variation: WT"	"characteristics_ch1.1"
"Strains were grown in LB at 37°C with shaking at 250 rpm.  16 h overnight cultures were diluted to OD600 = 0.05 in HEPES-glucose (0.4%), grown to OD600 ~ 0.6, and exposed to 4.5% deoxycholate for 30 min."	"growth_protocol_ch1.1"
"Escherichia coli BW25113"	"organism_ch1.1"
"BW25113 with deoxycholate stress"	"source_name_ch1.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice."	"treatment_protocol_ch1.1"