GSE58806-GSM1419897-GPL14649-PMID:.tsv
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"strain: GLBRCE1" "characteristics_ch1.1"
"medium: SynH_LT" "characteristics_ch1.2"
"growth phase: Stat2" "characteristics_ch1.3"
"biological replicate: 327" "characteristics_ch1.4"
"technical replicate: 1" "characteristics_ch1.5"
"Probe signal intensities were preprocessed using robust multichip averaging (RMA) in the program ArrayStar (DNASTAR), and the resulting gene expression signals were quantile-normalized across all samples using the normalize.quantiles function in the Bioconductor package for R." "data_processing.1"
"RNA was obtained from cells pellets by lysozyme treatment and phenol-chloroform extraction, analyzed by agarose gel electrophoresis to confirm integrity, quantified using a Nanodrop spectrophotometer (Thermo Scientific), and stored at -80°C" "extract_protocol_ch1.1"
"Fermentations were carried out in 3 L bioreactors (Applikon Biotechnology) containing 2.45 L of ACSH or SynH media, and cultures were diluted into ACSH or SynH with initial OD at 0.2, grown anaerobically overnight, and then inoculated into bioreactors to a starting OD600 of 0.2" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"GLBRCE1, SynH_LT, Stat2" "source_name_ch1.1"
"GLBRCE1_SynH_LT_Stat2_rep327_1" "title.1"
"Cells (10 ml) for transcriptomic analysis were collected into tubes containing 1.25 ml ice-cold 5% (vol/vol) unbuffered phenol in ethanol (EP) and pelleted by centrifugation (10,000 g, 4°C, 3 min). To remove residual traces of hydrolysate, cell pellets were twice resuspended in ice-cold GMM plus 0.125 volume of EP, repelleted, then flash frozen in dry ice-ethanol, and stored at -80°C." "treatment_protocol_ch1.1"
"strain: GLBRCE1" "characteristics_ch1.1"
"medium: SynH_LT" "characteristics_ch1.2"
"growth phase: Stat2" "characteristics_ch1.3"
"biological replicate: 327" "characteristics_ch1.4"
"technical replicate: 1" "characteristics_ch1.5"
"Fermentations were carried out in 3 L bioreactors (Applikon Biotechnology) containing 2.45 L of ACSH or SynH media, and cultures were diluted into ACSH or SynH with initial OD at 0.2, grown anaerobically overnight, and then inoculated into bioreactors to a starting OD600 of 0.2" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"GLBRCE1, SynH_LT, Stat2" "source_name_ch1.1"
"Cells (10 ml) for transcriptomic analysis were collected into tubes containing 1.25 ml ice-cold 5% (vol/vol) unbuffered phenol in ethanol (EP) and pelleted by centrifugation (10,000 g, 4°C, 3 min). To remove residual traces of hydrolysate, cell pellets were twice resuspended in ice-cold GMM plus 0.125 volume of EP, repelleted, then flash frozen in dry ice-ethanol, and stored at -80°C." "treatment_protocol_ch1.1"