"strain: wt 3xflag strain"	"characteristics_ch1.1"
"genotype: wild type"	"characteristics_ch1.2"
"chip antibody: anti-Flag (Sigma)"	"characteristics_ch1.3"
"Mapping was performed using BWA tool (version 0.5.9) against the reference genome"	"data_processing.1"
"Reference genome used: Escherichia coli K12 MG1655, version: iGenome"	"data_processing.2"
"Peak detection and count of coverage were done using SEQMONK version 0.21.0 by Fasteris SA"	"data_processing.3"
"Genome_build: Reference genome used: Escherichia coli K12 MG1655, version: iGenome"	"data_processing.4"
"Supplementary_files_format_and_content: .txt file file with raw and normalized counts of coverage"	"data_processing.5"
"Cross-linked cells were harvested and washed two times with ice-cold PBS. Cells were resuspended in 450μl of TES buffer (50mM Tris-HCl pH 7.5, 150mM NaCl) and 20μl of lysis solution (13.6mg/ml lysozyme, 50% glycerol, 50mM Tris-HCl pH7.5, 100mM NaCl, 1mM DTT, 0.1% Triton X-100) followed of a 5min incubation at room temperature. 10μl of cOmplete EDTA free protease inhibitor (Roche) were added and incubated 10min at room temperature. 550μl of ChIP buffer (1.1% Triton X-100, 1.2mM EDTA, 16.7mM Tris-HCl, 167mM NaCl, 20μl/ml of cOmplete EDTA free protease inhibitor (Roche) were added followed of 10min incubation at 37ºC. The lysate was then sonicated (UP200S-Hielscher) to an average size between 300bp and 700bp with 5 cycles of and amplitude of 55%, 0.45sec pulse during 10sec and 50sec in ice. Insoluble cell debris were removed by centrifugation at 20000g for 3min at 4ºC and the supernatant collected. The supernatant was added to α-Flag-agarose beads (Sigma) and incubated at 4ºC under rotation. The samples were then washed once with low salt wash buffer (0.1% SDS, 1% Triton X-100, 2mM EDTA, 20mM Tris-HCl pH 8.1, 150mM NaCl), once with high salt wash buffer (0.1% SDS, 1% Triton X-100, 2mM EDTA, 20mM Tris-HCl pH 8.1, 500mM NaCl), once with LiCl wash buffer (250mM LiCl, 1% NP-40, 1% deoxycholate, 1mM EDTA, 10mM Tris-HCl pH 8.1), and twice with TE buffer (10mM Tris-HCl pH 8.1, 1mM EDTA). Two washes with 250μl of freshly prepared elution buffer (1% SDS, 100mM NaHCO3) were done followed by vortexing and incubation under rotation at room temperature for 15min. To reverse the cross-link 30μl of 5M NaCl were added to the elute and incubated over night at 65ºC. Finally, 4μl of 0.5M EDTA, 20μl of 1M Tris-HCl pH 6.5 and 2μl of 10mg/ml Proteinase-K (Sigma) were added and the suspension incubated at 45ºC for 2h. DNA was purified and recovered by standard phenol-chloroform extraction and ethanol precipitation with 20μg of glycogen."	"extract_protocol_ch1.1"
"Library construction was performed by processing in vitro samples to generate a library of short inserts (the DNA Colonies Template Library) by Fasteris SA, Switzerland"	"extract_protocol_ch1.2"
"The ΔbolA and wt 3xflag strains were used to perform ChIP-seq experiments. Overnight cultures were diluted 1/100 in fresh LB medium and grown until OD600 0.6"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"bacterial cell culture"	"source_name_ch1.1"
"wild type"	"title.1"
"10 mM of sodium phosphate and formaldehyde to a final concentration of 1% were then added. After 10min of incubation at room temperature, the samples were incubated 30 min in ice"	"treatment_protocol_ch1.1"
"ChIP-Seq"	"library_strategy.1"
"strain: wt 3xflag strain"	"characteristics_ch1.1"
"genotype: wild type"	"characteristics_ch1.2"
"chip antibody: anti-Flag (Sigma)"	"characteristics_ch1.3"
"The ΔbolA and wt 3xflag strains were used to perform ChIP-seq experiments. Overnight cultures were diluted 1/100 in fresh LB medium and grown until OD600 0.6"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"bacterial cell culture"	"source_name_ch1.1"
"10 mM of sodium phosphate and formaldehyde to a final concentration of 1% were then added. After 10min of incubation at room temperature, the samples were incubated 30 min in ice"	"treatment_protocol_ch1.1"