GSE56251-GSM1357720-GPL3154-PMID:25028428.tsv 1.96 KB
"sample type: E. coli strain MC4100relA+ in mid log phase incubated at 37ºC for 4 hours with 100 μg/ml NA"	"characteristics_ch1.1"
"genotype: WT"	"characteristics_ch1.2"
"treatment: 100 μg/ml nalidixic acid"	"characteristics_ch1.3"
"RMA normalization was performed on CELL files using the Partek Genomic Suite 6.5. Additional statistical analysis was carried out using the Spotfire software package (Somerville, MA, USA) and custom Matlab (R2010B) routines."	"data_processing.1"
"Gene selection was done using the False Discovery Rate (FDR) correction procedure for adjusting genes with p-value smaller than 0.05 and Fold Change greater than 2."	"data_processing.2"
"We extracted RNA using RNAprotect Bacteria reagent (Qiagen) and RNeasy Mini Kit (Qiagen) combined with RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions."	"extract_protocol_ch1.1"
"We grew cells in 10 ml M9 minimal medium to OD600=0.5-0.6. Then, we divided each culture into 500µl aliquots to which we added the appropriate concentration of NA."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli strain MC4100relA+ in mid log phase incubated at 37ºC for 4 hours with 100 μg/ml NA"	"source_name_ch1.1"
"WT 100 μg/ml NA rep3"	"title.1"
"We incubated each aliquot at 37ºC for 4 hours with NA"	"treatment_protocol_ch1.1"
"sample type: E. coli strain MC4100relA+ in mid log phase incubated at 37ºC for 4 hours with 100 μg/ml NA"	"characteristics_ch1.1"
"genotype: WT"	"characteristics_ch1.2"
"treatment: 100 μg/ml nalidixic acid"	"characteristics_ch1.3"
"We grew cells in 10 ml M9 minimal medium to OD600=0.5-0.6. Then, we divided each culture into 500µl aliquots to which we added the appropriate concentration of NA."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli strain MC4100relA+ in mid log phase incubated at 37ºC for 4 hours with 100 μg/ml NA"	"source_name_ch1.1"
"We incubated each aliquot at 37ºC for 4 hours with NA"	"treatment_protocol_ch1.1"