"incubation medium: 10 mM NaCl + 100mg/l TiO2 nanoparticles"	"characteristics_ch1.1"
"incubation condition: In the dark, 5h, 20°C, 150 rpm"	"characteristics_ch1.2"
"Image analysis was performed with GenePix Pro 7 software (Molecular Device), background was defined with the “Local features background median” method. Quantile normalization was applied on the total number of spots in R (version 2.11.1) with the LIMMA software package. Spots corresponding to the E. coli MG1655 K12 species were extracted."	"data_processing.1"
"Total RNA was extracted from cells with the UltraClean Microbial RNA isolation kit (MO BIO, CA, USA). After extraction, contaminating DNA was digested with DNAse I (Sigma Aldrich) and total RNA was purified by phenol/chloroform extraction and ethanol precipitation. RNA purity and quantity was assess by optical density measurements (OD 260/280 and OD 260/230) and RNA integrity was checked with the Bioanalyseur 2100 (Agilent, CA, USA)."	"extract_protocol_ch1.1"
"Cell exposure to NP-TiO2 was conducted in 50 ml of sterile Milli-Q water supplemented with 10 mM NaCl. Briefly, 500 µl of the E. coli bacterial suspension and 500 µl of the NP-TiO2 stock suspension (or mQ water for the control) were prepared as previously described and then added to the NaCl solution to obtain final concentrations of 10E7 cells/ml and 100 mg/l of TiO2 nanoparticles. The flasks were then incubated at 20°C on a dark rotary shaker (150 rpm) for 5 h."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E.coli,  NP-TiO2 exposed culture, 5h in 10 mM NaCl 100mg/L NP-TiO2, replicate 3"	"source_name_ch1.1"
"Ecoli_NP-TiO2_5h_rep3"	"title.1"
"After 5 h of NP-TiO2 (or mQ water for the control) exposure, cells were incubated for 5 min with two volumes of RNAprotect Bacteria Reagent (Qiagen SAS, France) at room temperature. Cells were then pelleted by centrifugation (7,000 g, 10 min) and the pellet stored at -80°C."	"treatment_protocol_ch1.1"
"incubation medium: 10 mM NaCl + 100mg/l TiO2 nanoparticles"	"characteristics_ch1.1"
"incubation condition: In the dark, 5h, 20°C, 150 rpm"	"characteristics_ch1.2"
"Cell exposure to NP-TiO2 was conducted in 50 ml of sterile Milli-Q water supplemented with 10 mM NaCl. Briefly, 500 µl of the E. coli bacterial suspension and 500 µl of the NP-TiO2 stock suspension (or mQ water for the control) were prepared as previously described and then added to the NaCl solution to obtain final concentrations of 10E7 cells/ml and 100 mg/l of TiO2 nanoparticles. The flasks were then incubated at 20°C on a dark rotary shaker (150 rpm) for 5 h."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E.coli,  NP-TiO2 exposed culture, 5h in 10 mM NaCl 100mg/L NP-TiO2, replicate 3"	"source_name_ch1.1"
"After 5 h of NP-TiO2 (or mQ water for the control) exposure, cells were incubated for 5 min with two volumes of RNAprotect Bacteria Reagent (Qiagen SAS, France) at room temperature. Cells were then pelleted by centrifugation (7,000 g, 10 min) and the pellet stored at -80°C."	"treatment_protocol_ch1.1"