GSE55719-GSM1342413-GPL13336-PMID:25564530.tsv 1.95 KB
"strain: DH1 ΔhisG::cat ΔintC::Ptrc-dsred.t4-tetR-PEM7-zeo ΔgalK::PtetA-gfpuv5"	"characteristics_ch1.1"
"condition: histidine depleted"	"characteristics_ch1.2"
"Microarray data were processed using custom scripts written in R based on the finite hybridisation (FH) model (Ono et al, 2008) and the thermodynamic model of non-specific binding (NSB) on short nucleotide microarrays (Furusawa et al, 2009). The log10 mRNA concentration (pM) data are provided as a supplementary file on the SERIES record."	"data_processing.1"
"Total RNAs were extracted using an RNeasy mini kit (Qiagen) in accordance with the manufacturer’s instructions."	"extract_protocol_ch1.1"
"Cells were grown in the minimal media, modified M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"OSU12-hisG, histidine depleted, 2 hrs"	"source_name_ch1.1"
"OSU12-hisG, histidine depleted, 2 hrs, rep 3"	"title.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"
"strain: DH1 ΔhisG::cat ΔintC::Ptrc-dsred.t4-tetR-PEM7-zeo ΔgalK::PtetA-gfpuv5"	"characteristics_ch1.1"
"condition: histidine depleted"	"characteristics_ch1.2"
"Cells were grown in the minimal media, modified M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"OSU12-hisG, histidine depleted, 2 hrs"	"source_name_ch1.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"