"background strain: BW25113"	"characteristics_ch1.1"
"genotype/variation: fur-knockout mutant"	"characteristics_ch1.2"
"CHP files were generated using Affymetrix Expression Control.  Downstream data analysis was performed in GeneSpring GX 12,  CEL files were imported using RMA algorithm and median normalization was performed."	"data_processing.1"
"Samples for microarray analyses were taken before ciprofloxacin treatment (t0) and then at 1 hour post-treatment (t1). Cell concentration was adjusted to 109 cells/ml and cultures were treated with ciprofloxacin in Erlenmeyer flasks using 20 ml fresh LB. ~109 cells were taken at each timepoint as samples for RNA isolation. In order to stabilize RNA, RNA Bacteria Protect Reagent (Qiagen) was added to the samples according to the instructions of the manufacturer and then samples were stored overnight at -80°C. Total RNA was isolated by using RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. Sample concentration was estimated using the NanoDrop 1000 (Thermoscientific) spectrophotometer."	"extract_protocol_ch1.1"
"Escherichia coli BW25113 and fur mutant strains were cultivated at 37Co in 25 ml of LB medium using Erlenmeyer flasks. 1 ml of OVN culture was transferred to 20 ml of fresh LB medium and cells were incubated in the presence or absence of ciprofloxacin (100 ng/ml)."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"Cirpofloxacin treated fur mutant sample at T1h, biological rep 1"	"source_name_ch1.1"
"FUR_CPR_1"	"title.1"
"We compared the microarray-determined mRNA profiles (Affymetrix E. coli Genome 2.0 Array) of Δfur and WT genotypes in response to ciprofloxacin treatment (100 ng/ml) with those of untreated cultures. We used two biological replicates per genotype."	"treatment_protocol_ch1.1"
"background strain: BW25113"	"characteristics_ch1.1"
"genotype/variation: fur-knockout mutant"	"characteristics_ch1.2"
"Escherichia coli BW25113 and fur mutant strains were cultivated at 37Co in 25 ml of LB medium using Erlenmeyer flasks. 1 ml of OVN culture was transferred to 20 ml of fresh LB medium and cells were incubated in the presence or absence of ciprofloxacin (100 ng/ml)."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"Cirpofloxacin treated fur mutant sample at T1h, biological rep 1"	"source_name_ch1.1"
"We compared the microarray-determined mRNA profiles (Affymetrix E. coli Genome 2.0 Array) of Δfur and WT genotypes in response to ciprofloxacin treatment (100 ng/ml) with those of untreated cultures. We used two biological replicates per genotype."	"treatment_protocol_ch1.1"