GSE54900-GSM1326347-GPL17439-PMID:25222563.tsv
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"genotype/variation: WT" "characteristics_ch1.1"
"agent: Fe" "characteristics_ch1.2"
"Sequenced reads were mapped onto NC_000913 reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S" "data_processing.1"
"Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using cufflinks v.1.3.0" "data_processing.2"
"Genome_build: ASM584v2" "data_processing.3"
"Supplementary_files_format_and_content: Comma-delimited text file includes RPKM values for each Sample." "data_processing.4"
"Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a NanoDrop 1000 (Thermo Scientific Inc., Wilmington, DE, USA)." "extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols" "extract_protocol_ch1.2"
"E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Total RNA isolated from E. coli" "source_name_ch1.1"
"WT with Fe 1 (RNA-seq)" "title.1"
"The cell culture was treated with the RNAprotect reagent (Qiagen)." "treatment_protocol_ch1.1"
"RNA-Seq" "library_strategy.1"
"genotype/variation: WT" "characteristics_ch1.1"
"agent: Fe" "characteristics_ch1.2"
"E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Total RNA isolated from E. coli" "source_name_ch1.1"
"The cell culture was treated with the RNAprotect reagent (Qiagen)." "treatment_protocol_ch1.1"