GSE54899-GSM1326337-GPL17439-PMID:25222563.tsv 2.25 KB
"genotype/variation: fur-8myc"	"characteristics_ch1.1"
"chip antibody: anti-myc (Santa Cruz Biotech, sc-28207)"	"characteristics_ch1.2"
"agent: DPD"	"characteristics_ch1.3"
"library strategy: ChIP-exo"	"data_processing.1"
"ChIP-exo reads were aligned to the NC_000913 genome reference sequence using using bowtie v1.0.0 with parameters  -S"	"data_processing.2"
"Genome_build: ASM584v2"	"data_processing.3"
"Supplementary_files_format_and_content: Tab-delimited text files in gff format which has 8 columns: sequence id, source(empty), feature (+/- strand), start position, end position, intensity score, strand(+/-), frame(.), attribute(.)."	"data_processing.4"
"ChIP-seq libraries were prepared for sequencing using standard Illumina protocols"	"extract_protocol_ch1.1"
"E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Immunoprecipitated DNA"	"source_name_ch1.1"
"Fur with DPD 1 (ChIP-exo)"	"title.1"
"ChIP-Seq"	"library_strategy.1"
"genotype/variation: fur-8myc"	"characteristics_ch1.1"
"chip antibody: anti-myc (Santa Cruz Biotech, sc-28207)"	"characteristics_ch1.2"
"agent: DPD"	"characteristics_ch1.3"
"E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Immunoprecipitated DNA"	"source_name_ch1.1"