GSE54271-GSM1311347-GPL3154-PMID:24797297.tsv 1.32 KB
"genotype/variation: overexpression of arT (pCA24N_arT)"	"characteristics_ch1.1"
"Data was analyzed in the Affymetrix GenomeChipOperating Software (GCOS)."	"data_processing.1"
"Cells were lysed using a bead beater (Biospec) and mRNA was isolated using a Qiagen RNeasy mini kit (Cat# 74104)."	"extract_protocol_ch1.1"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm.  Overnight cultures were diluted into fresh LBCm30, grown for 2.5 h, and induced with 1 mM IPTG for 90 min (final OD600 = 1.6)."	"growth_protocol_ch1.1"
"Escherichia coli BW25113"	"organism_ch1.1"
"overexpression of arT (pCA24N_arT)"	"source_name_ch1.1"
"BW25113-pCA24N_arT LB 90 min 1 mM IPTG"	"title.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice."	"treatment_protocol_ch1.1"
"genotype/variation: overexpression of arT (pCA24N_arT)"	"characteristics_ch1.1"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm.  Overnight cultures were diluted into fresh LBCm30, grown for 2.5 h, and induced with 1 mM IPTG for 90 min (final OD600 = 1.6)."	"growth_protocol_ch1.1"
"Escherichia coli BW25113"	"organism_ch1.1"
"overexpression of arT (pCA24N_arT)"	"source_name_ch1.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice."	"treatment_protocol_ch1.1"