GSE54271-GSM1311347-GPL3154-PMID:24797297.tsv
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"genotype/variation: overexpression of arT (pCA24N_arT)" "characteristics_ch1.1"
"Data was analyzed in the Affymetrix GenomeChipOperating Software (GCOS)." "data_processing.1"
"Cells were lysed using a bead beater (Biospec) and mRNA was isolated using a Qiagen RNeasy mini kit (Cat# 74104)." "extract_protocol_ch1.1"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm. Overnight cultures were diluted into fresh LBCm30, grown for 2.5 h, and induced with 1 mM IPTG for 90 min (final OD600 = 1.6)." "growth_protocol_ch1.1"
"Escherichia coli BW25113" "organism_ch1.1"
"overexpression of arT (pCA24N_arT)" "source_name_ch1.1"
"BW25113-pCA24N_arT LB 90 min 1 mM IPTG" "title.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice." "treatment_protocol_ch1.1"
"genotype/variation: overexpression of arT (pCA24N_arT)" "characteristics_ch1.1"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm. Overnight cultures were diluted into fresh LBCm30, grown for 2.5 h, and induced with 1 mM IPTG for 90 min (final OD600 = 1.6)." "growth_protocol_ch1.1"
"Escherichia coli BW25113" "organism_ch1.1"
"overexpression of arT (pCA24N_arT)" "source_name_ch1.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice." "treatment_protocol_ch1.1"