"treatment: bicontinious microemulsion"	"characteristics_ch1.1"
"strain: CMCC(B) 44102"	"characteristics_ch1.2"
"Raw microarray intensity data were normalized per chip to the same mean value, which is recommended by the manufacturer."	"data_processing.1"
"Total RNA from cells of the microemulsion-treated E. coli was extracted in duplicate using an RNAiso Plus (Cat. # 908, Takara, Japan) according to the manufacturer’s instructions,"	"extract_protocol_ch1.1"
"The strain E. coli CMCC(B) 44102, was provided by Institute of Microbiology, Chinese Academy of Sciences and preserved at the Department of Food Science and Nutrition, Zhejiang University. The strain was maintained in nutrient agar medium (NA, Hangzhou Microbiological Agents Co., Ltd., China) at 37 °C, and cultured in tryptic soy broth (TSB, Qingdao Hope Biotechnology Co., Ltd., China) at pH 7.0 and transferred every 20-24 h with incubation at 37 °C."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"cells"	"source_name_ch1.1"
"E. coli treated with bicontinious microemulsion"	"title.1"
"5 ml bacterial cultures with a known inoculum of E. coli cells (1.0 × 109 cfu/ml) was added at 1:1 (v/v) to the microemulsion and incubated on a tube rotator at 200 rpm for 4 h at 37 °C for the subsequent DNA microarray analysis. In the preliminary experiment, a complete loss of E. coli viability was achieved after 8 h by the aforementioned treatment. The prepared microemulsion has an oil phase of glycerol monolaurate (5.5% w/w of the total emulsion), ethanol (5.5%), and a surfactant phase of Tween 20 (43%). Mixtures of the surfactant-oil phase were prepared in stoppered flasks and kept in a 25 °C water bath. Subsequently, the water phase was added, and the mixture was treated in an ultrasonic bath until the system became optically clear, and allowed to equilibrate at 25 °C for at least 24 h to guarantee steady-state conditions."	"treatment_protocol_ch1.1"
"treatment: bicontinious microemulsion"	"characteristics_ch1.1"
"strain: CMCC(B) 44102"	"characteristics_ch1.2"
"The strain E. coli CMCC(B) 44102, was provided by Institute of Microbiology, Chinese Academy of Sciences and preserved at the Department of Food Science and Nutrition, Zhejiang University. The strain was maintained in nutrient agar medium (NA, Hangzhou Microbiological Agents Co., Ltd., China) at 37 °C, and cultured in tryptic soy broth (TSB, Qingdao Hope Biotechnology Co., Ltd., China) at pH 7.0 and transferred every 20-24 h with incubation at 37 °C."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"cells"	"source_name_ch1.1"
"5 ml bacterial cultures with a known inoculum of E. coli cells (1.0 × 109 cfu/ml) was added at 1:1 (v/v) to the microemulsion and incubated on a tube rotator at 200 rpm for 4 h at 37 °C for the subsequent DNA microarray analysis. In the preliminary experiment, a complete loss of E. coli viability was achieved after 8 h by the aforementioned treatment. The prepared microemulsion has an oil phase of glycerol monolaurate (5.5% w/w of the total emulsion), ethanol (5.5%), and a surfactant phase of Tween 20 (43%). Mixtures of the surfactant-oil phase were prepared in stoppered flasks and kept in a 25 °C water bath. Subsequently, the water phase was added, and the mixture was treated in an ultrasonic bath until the system became optically clear, and allowed to equilibrate at 25 °C for at least 24 h to guarantee steady-state conditions."	"treatment_protocol_ch1.1"