GSE49296-GSM1196966-GPL13336-PMID:24252326.tsv 1.73 KB
"strain: MDS42"	"characteristics_ch1.1"
"condition: osmotic pressure"	"characteristics_ch1.2"
"Microarray data were processed using custom scripts written in R based on the finite hybridisation (FH) model (Ono et al, 2008) and the thermodynamic model of non-specific binding (NSB) on short nucleotide microarrays (Furusawa et al, 2009)."	"data_processing.1"
"Total RNAs were extracted using an RNeasy mini kit (Qiagen) in accordance with the manufacturer’s instructions."	"extract_protocol_ch1.1"
"Cells were grown in the minimal media, M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli MDS42, osmotic pressure, NaCl 0.45 M"	"source_name_ch1.1"
"E. coli MDS42, osmotic pressure, NaCl 0.45 M, rep 1"	"title.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"
"strain: MDS42"	"characteristics_ch1.1"
"condition: osmotic pressure"	"characteristics_ch1.2"
"Cells were grown in the minimal media, M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli MDS42, osmotic pressure, NaCl 0.45 M"	"source_name_ch1.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"