GSE47427-GSM1149311-GPL3154-PMID:25219496.tsv 1.39 KB
"strain: BW25113"	"characteristics_ch1.1"
"genotype/variation: overexpression of dosP (pCA24N_dosP)"	"characteristics_ch1.2"
"Data was analyzed in the Affymetrix GenomeChipOperating Software (GCOS)."	"data_processing.1"
"Cells were lysed using a bead beater (Biospec) and mRNA was isolated using a Qiagen RNeasy mini kit (Cat# 74104)."	"extract_protocol_ch1.1"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm.  16 h overnight cultures were diluted at 1:1000 into fresh LBCm30, grown to OD600 ~ 1.0, and induced with 1 mM IPTG for 90 min."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"overexpression of dosP (pCA24N_dosP)"	"source_name_ch1.1"
"BW25113-pCA24N_dosP"	"title.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice."	"treatment_protocol_ch1.1"
"strain: BW25113"	"characteristics_ch1.1"
"genotype/variation: overexpression of dosP (pCA24N_dosP)"	"characteristics_ch1.2"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm.  16 h overnight cultures were diluted at 1:1000 into fresh LBCm30, grown to OD600 ~ 1.0, and induced with 1 mM IPTG for 90 min."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"overexpression of dosP (pCA24N_dosP)"	"source_name_ch1.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice."	"treatment_protocol_ch1.1"