GSE4724-GSM106752-GPL3154-PMID:17074904.tsv 1.77 KB
"E.Coli, strain P4X, grown on minimal medium, reference sample"	"characteristics_ch1.1"
"Initial data analysis was performed using the GeneChip® Operating Software. Microarray quality control parameters were as follow: noise (RawQ) less than 5, background signal less than 40 (100 target intensity for array scaling), consistent numbers of genes detected as present across arrays."	"data_processing.1"
"Total RNA was isolated using a RNeasy® RNA isolation kit, according to the manufacturer’s specifications (QIAGEN, Germany) and stored in RNase-free water at –80°C. RNA concentration was determined by UV-spectrometry and its quality controlled by agarose gel electrophoresis."	"extract_protocol_ch1.1"
"Cells used for expression analysis were grown in minimal medium (Glansdorff, 1965) supplemented with 0.5% glucose (w/v), L-methionine (100ug ml-1). For expression analysis, cells were grown in a rotary shaker at 37°C and harvested by centrifugation at mid-log phase ( (OD) 660nm = 0.5) and the metabolism was quenched in liquid nitrogen."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.Coli, strain P4X, grown on minimal medium, reference sample"	"source_name_ch1.1"
"P4X Wild Type (1989) Reference"	"title.1"
"E.Coli, strain P4X, grown on minimal medium, reference sample"	"characteristics_ch1.1"
"Cells used for expression analysis were grown in minimal medium (Glansdorff, 1965) supplemented with 0.5% glucose (w/v), L-methionine (100ug ml-1). For expression analysis, cells were grown in a rotary shaker at 37°C and harvested by centrifugation at mid-log phase ( (OD) 660nm = 0.5) and the metabolism was quenched in liquid nitrogen."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.Coli, strain P4X, grown on minimal medium, reference sample"	"source_name_ch1.1"