GSE46455-GSM1130593-GPL13359-PMID:25250024.tsv 2.35 KB
"strain background: B"	"characteristics_ch1.1"
"genotype/variation: wild type"	"characteristics_ch1.2"
"Data quantification was performed using Agilent Feature Extraction software 9.3.2.1 (Agilent Technology, USA). The average fluorescence intensity for each spot was calculated and local background was subtracted. All data normalization and selection of fold-changed genes were performed using GeneSpringGX 7.3.1 (Agilent Technology, USA). Normalization for Agilent one-color method was performed, which is Data transformation. Set measurements less than 5.0 to 5.0 and Per Chip : Normalize to 50th percentage. Reliable genes were filtered by flag as following the Agilent manual."	"data_processing.1"
"For control and test RNAs, the synthesis of target cRNA probes and hybridization were performed using Agilent’s Low Input Quick Amp WT Labeling Kit, one color (Agilent Technology, USA) according to the manufacturer’s instructions. Briefly, each 0.2ug total RNA was mixed with WT primer mix and incubated at 65ºC for 10min. cDNA master mix (5X First strand buffer, 0.1M DTT, 10mM dNTP mix, RNase-Out, and MMLV-RT) was prepared and added to the reaction mixer. The samples were incubated at 40ºC for 2 hours and then the RT and dsDNA synthesis was terminated by incubating at 70ºC for 15min."	"extract_protocol_ch1.1"
"The E. coli strains were grown for six hours under anaerobic growth condition in glucose-containing complex medium."	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"B strain_wild type"	"source_name_ch1.1"
"B strain, wild type"	"title.1"
"The microarray experiment was performed with two serotypes of wild type E. coli and their adhE mutants: (1) K-12 strain, wild type; (2) B strain, wild type; (3) BW25113, adhE mutants; (4) BL21(DE3), adhE mutants."	"treatment_protocol_ch1.1"
"strain background: B"	"characteristics_ch1.1"
"genotype/variation: wild type"	"characteristics_ch1.2"
"The E. coli strains were grown for six hours under anaerobic growth condition in glucose-containing complex medium."	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"B strain_wild type"	"source_name_ch1.1"
"The microarray experiment was performed with two serotypes of wild type E. coli and their adhE mutants: (1) K-12 strain, wild type; (2) B strain, wild type; (3) BW25113, adhE mutants; (4) BL21(DE3), adhE mutants."	"treatment_protocol_ch1.1"