"sample type: mock community [artificial bacterial DNA mix]"	"characteristics_ch1.1"
"Pixel intensities were extracted using the “Feature Extraction” software (Agilent Technologies, Palo Alto, CA). No normalization step was performed and the retained intensity value for each probe was the ratio between the spot’s median intensity signals and the median of background signals."	"data_processing.1"
"Total DNA was extracted from three human faecal samples using  Qiagen’s DNA Stool Kit (Qiagen, West Sussex, UK). Total genomic DNA was extracted from pure bacterial cultures using DNeasy Blood and Tissue Kit (Qiagen, West Sussex, UK). 10ng of sample was then used for PCR amplification. 16S rRNA genes were amplified using universal primers 27F (AGAGTTTGATCMTGGCTCAG) and 1492R (TACGGYTACCTTGTTACGACT). PCR reactions were performed in a 50µl volume, using DreamTaq DNA polymerase (Fermentas, St. Leon-Rot, Germany). The PCR reaction consisted of an initial denaturation step at 95°C for 5min followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 58°C for 40 s and elongation at 72°C for 2 min. A final extension step was performed at 72°C for 5 min. PCR product size was verified by electrophoresis with 1% (w/v) agarose gel and were purified using the MinElute PCR Purification Kit (Qiagen Ltd., UK) following manufacturer’s instructions and stored at -20°C."	"extract_protocol_ch1.1"
"Faecal samples were collected by the Department of Microbiology and Alimentary Pharmabiotic Centre (Cork, Ireland) from three elderly patients (176, 204 and 205). The mock community was performed after culture of each individual bacterial strain in their reference medium at their optimal temperature for several days."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Bacteroides fragilis"	"organism_ch1.2"
"Blautia coccoides"	"organism_ch1.3"
"[Clostridium] leptum"	"organism_ch1.4"
"Lactobacillus acidophilus"	"organism_ch1.5"
"Mock community"	"source_name_ch1.1"
"100ng 16S rRNA gene amplicon of a mock community"	"title.1"
"No treatment were studied in this experience"	"treatment_protocol_ch1.1"
"sample type: mock community [artificial bacterial DNA mix]"	"characteristics_ch1.1"
"Faecal samples were collected by the Department of Microbiology and Alimentary Pharmabiotic Centre (Cork, Ireland) from three elderly patients (176, 204 and 205). The mock community was performed after culture of each individual bacterial strain in their reference medium at their optimal temperature for several days."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Bacteroides fragilis"	"organism_ch1.2"
"Blautia coccoides"	"organism_ch1.3"
"[Clostridium] leptum"	"organism_ch1.4"
"Lactobacillus acidophilus"	"organism_ch1.5"
"Mock community"	"source_name_ch1.1"
"No treatment were studied in this experience"	"treatment_protocol_ch1.1"