GSE44258-GSM1081386-GPL13359-PMID:.tsv
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"plasmid source: Post Selection Library" "characteristics_ch1.1"
"plasmid: Initial Recipient Library (BWG)" "characteristics_ch2.1"
"Data were extracted in GenePix 4100A scanning software and normalized using the lowess method implemented in TM4 MIDAS" "data_processing.1"
"Library minipreps were performed with the Zyppy Miniprep kit (Zymo Technologies) and quantified using the Qubit dsDNA BR kit (Invitrogen) and the Nanodrop spectrophotometer (Thermo Scientific)" "extract_protocol_ch1.1"
"Library minipreps were performed with the Zyppy Miniprep kit (Zymo Technologies) and quantified using the Qubit dsDNA BR kit (Invitrogen) and the Nanodrop spectrophotometer (Thermo Scientific)" "extract_protocol_ch2.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well." "growth_protocol_ch1.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well." "growth_protocol_ch2.1"
"Escherichia coli BW25113" "organism_ch1.1"
"Escherichia coli BW25113" "organism_ch2.1"
"Plasmid DNA, after n-butanol selection" "source_name_ch1.1"
"Plasmid DNA, BWG post conjugation" "source_name_ch2.1"
"BWG_butanol_3passages" "title.1"
"plasmid source: Post Selection Library" "characteristics_ch1.1"
"plasmid: Initial Recipient Library (BWG)" "characteristics_ch2.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well." "growth_protocol_ch1.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well." "growth_protocol_ch2.1"
"Escherichia coli BW25113" "organism_ch1.1"
"Escherichia coli BW25113" "organism_ch2.1"
"Plasmid DNA, after n-butanol selection" "source_name_ch1.1"
"Plasmid DNA, BWG post conjugation" "source_name_ch2.1"