GSE44258-GSM1081382-GPL13359-PMID:.tsv 2.94 KB
"plasmid source: Initial Recipient Library"	"characteristics_ch1.1"
"plasmid: Donor Library"	"characteristics_ch2.1"
"Data were extracted in GenePix 4100A scanning software and normalized using the lowess method implemented in TM4 MIDAS"	"data_processing.1"
"Library minipreps were performed with the Zyppy Miniprep kit (Zymo Technologies) and quantified using the Qubit dsDNA BR kit (Invitrogen) and the Nanodrop spectrophotometer (Thermo Scientific)"	"extract_protocol_ch1.1"
"Library minipreps were performed with the Zyppy Miniprep kit (Zymo Technologies) and quantified using the Qubit dsDNA BR kit (Invitrogen) and the Nanodrop spectrophotometer (Thermo Scientific)"	"extract_protocol_ch2.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well."	"growth_protocol_ch1.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well."	"growth_protocol_ch2.1"
"Escherichia coli BW25113"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Plasmid DNA, post conjugation"	"source_name_ch1.1"
"Plasmid DNA, donor, XL1-Blue"	"source_name_ch2.1"
"BWG_replicated"	"title.1"
"plasmid source: Initial Recipient Library"	"characteristics_ch1.1"
"plasmid: Donor Library"	"characteristics_ch2.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well."	"growth_protocol_ch1.1"
"Donor was transformed with a random genomic library made from E. coli and stored as glycerol stocks. The library was revived and transferred to four recipient strains using conjugation; the donor was miniprepped at this time, and the recipients were miniprepped after outgrowth under dual selection. Two recipient libraries in BWG and BWY were propagated under n-butanol stress for three days, and then miniprepped as well."	"growth_protocol_ch2.1"
"Escherichia coli BW25113"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Plasmid DNA, post conjugation"	"source_name_ch1.1"
"Plasmid DNA, donor, XL1-Blue"	"source_name_ch2.1"