"strain: T3 5H5 M"	"characteristics_ch1.1"
"genotype: triclosan tolerant mutant"	"characteristics_ch1.2"
"genetic background: O157 H19"	"characteristics_ch1.3"
"treatment: untreated"	"characteristics_ch1.4"
"Microarray analysis including pre-processing, normalisation anmd statistical analysis were performed using R (R, 2007) version 2.6 and Bioconductor (Gentleman et al. 2004, Genome Biol. 5:R80) version 2.1 as previously described by Morris et al.(2009, Physiol. Genomics 39:28-37). Propagating uncertainty in microarray analysis (puma) method was used to estimate fold changes and P-like values of differential gene expression analysis (Pearson et al. 2009, BMC Bioinformatics 10:211)."	"data_processing.1"
"Total RNA was stabilised and subsequently purified from a 500 ul portion of each bacterial culture using a RNAprotect Bacterial Reagent Mini Kit (Qiagen, Crawley, UK)"	"extract_protocol_ch1.1"
"The wildtype and mutant E. coli O157:H19 strains were grown on LB agar incubated at 37c x 24h. A single isolated colony for each strain was selected and inoculated into 20 ml LB broth and incubated at 37c until growth reached an optical density of 0.6 at 600nm."	"growth_protocol_ch1.1"
"Escherichia coli O157"	"organism_ch1.1"
"Mutant E. coli O157 untreated, biological rep 1"	"source_name_ch1.1"
"Triclosan tolerant mutant untreated biological rep 1"	"title.1"
"The subculture was split into two 10 ml portions, one of which was treated with 6ug/ml triclosan and the other retained as an untreated control. Treated and untreated samples were incubated at 37c for 30 min."	"treatment_protocol_ch1.1"
"strain: T3 5H5 M"	"characteristics_ch1.1"
"genotype: triclosan tolerant mutant"	"characteristics_ch1.2"
"genetic background: O157 H19"	"characteristics_ch1.3"
"treatment: untreated"	"characteristics_ch1.4"
"The wildtype and mutant E. coli O157:H19 strains were grown on LB agar incubated at 37c x 24h. A single isolated colony for each strain was selected and inoculated into 20 ml LB broth and incubated at 37c until growth reached an optical density of 0.6 at 600nm."	"growth_protocol_ch1.1"
"Escherichia coli O157"	"organism_ch1.1"
"Mutant E. coli O157 untreated, biological rep 1"	"source_name_ch1.1"
"The subculture was split into two 10 ml portions, one of which was treated with 6ug/ml triclosan and the other retained as an untreated control. Treated and untreated samples were incubated at 37c for 30 min."	"treatment_protocol_ch1.1"