GSE37026-GSM909098-GPL3154-PMID:23421615.tsv
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"treatment: treated culture" "characteristics_ch1.1"
"Data were normalized using RMA algorithm from R/Bioconductor/XPS package." "data_processing.1"
"Total RNA was extracted using RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol." "extract_protocol_ch1.1"
"Overnight cultures of E. coli MG1655 grown at 37°C with shaking at 200 rpm were diluted 1:625 into 50 ml of LB broth (Roth), and incubated at 37°C with shaking at 200 rpm." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Escherichia coli MG1655 after 60 min incubation following colicin M treatment" "source_name_ch1.1"
"treated sample at 60 min, biological rep1" "title.1"
"When the cultures reached an OD600 0.6 they were split into two flasks, each containing 20 ml of the culture. One was treated with a subinhibitory concentration of purified colicin M, while the untreated served as a control. 2-ml culture aliquotes were harvested from each flask after 30 min and 60 min incubation at 37°C and were mixed with 4 ml RNAProtect Bacteria Reagent (Qiagen)." "treatment_protocol_ch1.1"
"treatment: treated culture" "characteristics_ch1.1"
"Overnight cultures of E. coli MG1655 grown at 37°C with shaking at 200 rpm were diluted 1:625 into 50 ml of LB broth (Roth), and incubated at 37°C with shaking at 200 rpm." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Escherichia coli MG1655 after 60 min incubation following colicin M treatment" "source_name_ch1.1"
"When the cultures reached an OD600 0.6 they were split into two flasks, each containing 20 ml of the culture. One was treated with a subinhibitory concentration of purified colicin M, while the untreated served as a control. 2-ml culture aliquotes were harvested from each flask after 30 min and 60 min incubation at 37°C and were mixed with 4 ml RNAProtect Bacteria Reagent (Qiagen)." "treatment_protocol_ch1.1"