GSE34275-GSM846193-GPL3154-PMID:24858919.tsv 1.45 KB
"strain: Escherichia coli"	"characteristics_ch1.1"
"growth medium: LB medium"	"characteristics_ch1.2"
"The intensity cell files were then imported, normalized for background correction using PLIER and data analysed using Gene Spring 11.5 software."	"data_processing.1"
"RNA was isolated from the culture by using the Qiagen RNeasy mini-prep kit.cDNA was synthesized using 5-10 µg/ml of RNA and the first strand cDNA synthesis kit from Invitrogen (Invitrogen Bioservices India Pvt. Ltd., Bangalore)."	"extract_protocol_ch1.1"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth at 30°C in an incubator shaker at 150 r.p.m."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli, grown in LB to0.8OD"	"source_name_ch1.1"
"control, biological rep2 C4"	"title.1"
"To study gene expression in the presence and absence of glycerol, LB was supplemented with 10% (v/v) glycerol. Medium without glycerol served as a control."	"treatment_protocol_ch1.1"
"strain: Escherichia coli"	"characteristics_ch1.1"
"growth medium: LB medium"	"characteristics_ch1.2"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth at 30°C in an incubator shaker at 150 r.p.m."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli, grown in LB to0.8OD"	"source_name_ch1.1"
"To study gene expression in the presence and absence of glycerol, LB was supplemented with 10% (v/v) glycerol. Medium without glycerol served as a control."	"treatment_protocol_ch1.1"