GSE34275-GSM846192-GPL3154-PMID:24858919.tsv
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"strain: Escherichia coli" "characteristics_ch1.1"
"growth medium: LB medium" "characteristics_ch1.2"
"The intensity cell files were then imported, normalized for background correction using PLIER and data analysed using Gene Spring 11.5 software." "data_processing.1"
"RNA was isolated from the culture by using the Qiagen RNeasy mini-prep kit.cDNA was synthesized using 5-10 µg/ml of RNA and the first strand cDNA synthesis kit from Invitrogen (Invitrogen Bioservices India Pvt. Ltd., Bangalore)." "extract_protocol_ch1.1"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth at 30°C in an incubator shaker at 150 r.p.m." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E.coli, grown in LB to0.8OD" "source_name_ch1.1"
"control, biological rep1 C3" "title.1"
"To study gene expression in the presence and absence of glycerol, LB was supplemented with 10% (v/v) glycerol. Medium without glycerol served as a control." "treatment_protocol_ch1.1"
"strain: Escherichia coli" "characteristics_ch1.1"
"growth medium: LB medium" "characteristics_ch1.2"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth at 30°C in an incubator shaker at 150 r.p.m." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E.coli, grown in LB to0.8OD" "source_name_ch1.1"
"To study gene expression in the presence and absence of glycerol, LB was supplemented with 10% (v/v) glycerol. Medium without glycerol served as a control." "treatment_protocol_ch1.1"