"sample id: 2b"	"characteristics_ch1.1"
"genotype: Δ(yjjP-yjjQ-bglJ)"	"characteristics_ch1.2"
"strain: T75"	"characteristics_ch1.3"
"overexpression: -"	"characteristics_ch1.4"
"plasmid: pKESK22"	"characteristics_ch1.5"
"Data were processed using Affymetrix apt-probeset-summarize software version 1.10 and RMA algorithm. Samples were normalized using the standard normalization probes present on the Affymetrix GeneChip."	"data_processing.1"
"Cells were harvested using RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) for stabilization of RNA. Stabilized cultures were used for RNA isolation using the RNeasy MiniKit system (Qiagen, Hilden, Germany). In brief, 1 ml of each culture was used and processed according to the manufacturer's instructions including an on-column DNaseI treatment. RNA quality was assayed by denaturing urea-PAGE and by measuring the ratio of absorption at 260/280 nm in a GeneQuant II spectrophotometer (Amersham). RNA concentration was determined by measuring UV light absorption at 260 nm."	"extract_protocol_ch1.1"
"Exponential cultures of transformants were inoculated from fresh overnight cultures in LB medium supplemented with 25 µg/ml of kanamycin to an optical density at 600 nm (OD) of 0.1 and grown for 30 min at 37°C to an OD of 0.15. Then, IPTG was added to a final concentration of 1 mM for induction of expression. After 60 min of additional growth cells were harvested for RNA isolation."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"K12 Δ(yjjP-yjjQ-bglJ) + ctrl"	"source_name_ch1.1"
"K12 Δ(yjjP-yjjQ-bglJ) + ctrl, biological replicate 2"	"title.1"
"sample id: 2b"	"characteristics_ch1.1"
"genotype: Δ(yjjP-yjjQ-bglJ)"	"characteristics_ch1.2"
"strain: T75"	"characteristics_ch1.3"
"overexpression: -"	"characteristics_ch1.4"
"plasmid: pKESK22"	"characteristics_ch1.5"
"Exponential cultures of transformants were inoculated from fresh overnight cultures in LB medium supplemented with 25 µg/ml of kanamycin to an optical density at 600 nm (OD) of 0.1 and grown for 30 min at 37°C to an OD of 0.15. Then, IPTG was added to a final concentration of 1 mM for induction of expression. After 60 min of additional growth cells were harvested for RNA isolation."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"K12 Δ(yjjP-yjjQ-bglJ) + ctrl"	"source_name_ch1.1"