GSE30441-GSM754996-GPL3154-PMID:22792156.tsv 1.6 KB
"strain: DH5a"	"characteristics_ch1.1"
"genotype: ethanol-tolerant mutant E1 of IrrE"	"characteristics_ch1.2"
"Data were analyzed using GeneChip Operating Software Version 1.4 (Affymetrix)"	"data_processing.1"
"Total RNA was isolated from the cells stored in liquid nitrogen using TRIzol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and RNeasy MinElute Cleanup Kits (Qiagen, Valencia, CA, USA)"	"extract_protocol_ch1.1"
"Cells were grown at 37°C in Luria-Bertani (LB) medium containing 50 μg/ml ampicillin overnight, then diluted at a ratio of 1:100 into LB medium containing 1.5% (v/v) ethanol and 50 μg/ml ampicillin, and grown at 37°C."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Cells in log phase under ethanol stress"	"source_name_ch1.1"
"mutant E1, biological rep2"	"title.1"
"The cells were centrifuged when they reached the log phase, and then rapidly chilled and stored in liquid nitrogen until used for the DNA microarray assay"	"treatment_protocol_ch1.1"
"strain: DH5a"	"characteristics_ch1.1"
"genotype: ethanol-tolerant mutant E1 of IrrE"	"characteristics_ch1.2"
"Cells were grown at 37°C in Luria-Bertani (LB) medium containing 50 μg/ml ampicillin overnight, then diluted at a ratio of 1:100 into LB medium containing 1.5% (v/v) ethanol and 50 μg/ml ampicillin, and grown at 37°C."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Cells in log phase under ethanol stress"	"source_name_ch1.1"
"The cells were centrifuged when they reached the log phase, and then rapidly chilled and stored in liquid nitrogen until used for the DNA microarray assay"	"treatment_protocol_ch1.1"