"serotype: O157:H7"	"characteristics_ch1.1"
"strain: EDL933"	"characteristics_ch1.2"
"MAS 5.0 Expression Analysis Default Setting"	"data_processing.1"
"E. coli O157:H7 EDL933 was inoculated in 100 ml of LB in 250 ml shake flasks with overnight cultures that were diluted 1:100. Cells were shaken with 4 g of glass wool at 250 rpm and 37°C for 7 hrs.  Cells were immediately chilled with dry ice and 95% ethanol (to prevent RNA degradation) for 30 sec before centrifugation in 50 ml centrifuge tubes at 13,000 g for 2 min; cell pellets were frozen immediately with dry ice and stored -80°C.  RNA was isolated using Qiagen RNeasy mini Kit (Cat# 74104) with Qiagen RNase-free DNase I (Cat# 79254)."	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"RNA extracted from e. coli o157: H7 EDL933 cells grown (7 hrs incubation) in LB at 37ºC with DMSO"	"source_name_ch1.1"
"E. coli O157:H7 EDL933 in LB at 37oC for 7 hrs incubation with DMSO"	"title.1"
"serotype: O157:H7"	"characteristics_ch1.1"
"strain: EDL933"	"characteristics_ch1.2"
"Escherichia coli"	"organism_ch1.1"
"RNA extracted from e. coli o157: H7 EDL933 cells grown (7 hrs incubation) in LB at 37ºC with DMSO"	"source_name_ch1.1"