GSE28399-GSM701939-GPL3154-PMID:23090957.tsv 2.49 KB
"growth condition: 50 µl into K-medium + 0.3M NaCl"	"characteristics_ch1.1"
"GCOS was used to obtain detection calls for each microarray.  The statistical difference for mRNA expression were analyzed using CarmaWeb. Affymetrix GeneChip analysis was performed using MAS for background correction, VSN for normalization, MAS for PM correction and Medianpolish for Expression.  VSN normalized data not provided by submitter."	"data_processing.1"
"Total RNA was extracted by the hot phenol–chloroform method."	"extract_protocol_ch1.1"
"Cells from single colonies were grown on LB agar and then inoculated into liquid LB and grown to saturation. Then 50 µl was inoculated into 5 ml of K-medium and grown to saturation again. The cultures were then inoculated into the separate media as follows: 25 μl into K-medium, 50 µl into K-medium + 0.3M NaCl, 75 µl into K-medium + 0.6M urea; the final volume was 5ml in all cases. These cultures were grown overnight. The cultures were incubated in 250 ml Erlenmeyer flasks in the three media in a water bath at 30ºC."	"growth_protocol_ch1.1"
"Escherichia coli CFT073"	"organism_ch1.1"
"CFT073 in NaCl"	"source_name_ch1.1"
"CFT073 NaCl biological rep 2"	"title.1"
"When the samples had reached an OD600 of 0.4-0.5, 25 ml of each culture was added to a 2.5 ml mixture of cold 95% ethanol and 5% phenol to preserve the mRNA. The cells were cooled rapidly on ice and immediately harvested by centrifugation at 4°C, and the pellet was frozen on dry ice."	"treatment_protocol_ch1.1"
"growth condition: 50 µl into K-medium + 0.3M NaCl"	"characteristics_ch1.1"
"Cells from single colonies were grown on LB agar and then inoculated into liquid LB and grown to saturation. Then 50 µl was inoculated into 5 ml of K-medium and grown to saturation again. The cultures were then inoculated into the separate media as follows: 25 μl into K-medium, 50 µl into K-medium + 0.3M NaCl, 75 µl into K-medium + 0.6M urea; the final volume was 5ml in all cases. These cultures were grown overnight. The cultures were incubated in 250 ml Erlenmeyer flasks in the three media in a water bath at 30ºC."	"growth_protocol_ch1.1"
"Escherichia coli CFT073"	"organism_ch1.1"
"CFT073 in NaCl"	"source_name_ch1.1"
"When the samples had reached an OD600 of 0.4-0.5, 25 ml of each culture was added to a 2.5 ml mixture of cold 95% ethanol and 5% phenol to preserve the mRNA. The cells were cooled rapidly on ice and immediately harvested by centrifugation at 4°C, and the pellet was frozen on dry ice."	"treatment_protocol_ch1.1"