GSE28210-GSM698499-GPL13336-PMID:21613982.tsv 2.33 KB
"genotype: DH1 ΔhisC::cat ΔintC::Ptrc-rfp-tetR-zeocinr ΔgalK::PtetA-gfp-hisC-kanr"	"characteristics_ch1.1"
"platform_id design: EcFS_1"	"characteristics_ch1.2"
"Microarray data were processed using custom scripts written in R based on the finite hybridisation (FH) model (Ono et al (2008) An improved physico-chemical model of hybridization on high-density oligonucleotide microarrays. Bioinformatics 24: 1278-1285.) and the thermodynamic model of non-specific binding (NSB) on short nucleotide microarrays (Furusawa et al, 2009)."	"data_processing.1"
"log10 mRNA concentration (pM) data are provided as a supplementary file on the SERIES record."	"data_processing.2"
"Total RNAs were extracted using an RNeasy mini kit (Qiagen) in accordance with the manufacturer’s instructions."	"extract_protocol_ch1.1"
"Cells were transferred to the fresh minimal media, mM63, in the presence or absence of histidine. The initial cell concentrations were controlled as 104-105 cells/mL, and the final ones ~ 108 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"HisC rewired strain exponentially grown in the presence of histidine (1 mM)"	"source_name_ch1.1"
"OSU12-hisC, nurishment, biological rep 3"	"title.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"
"genotype: DH1 ΔhisC::cat ΔintC::Ptrc-rfp-tetR-zeocinr ΔgalK::PtetA-gfp-hisC-kanr"	"characteristics_ch1.1"
"platform_id design: EcFS_1"	"characteristics_ch1.2"
"Cells were transferred to the fresh minimal media, mM63, in the presence or absence of histidine. The initial cell concentrations were controlled as 104-105 cells/mL, and the final ones ~ 108 cells/mL within the exponential growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"HisC rewired strain exponentially grown in the presence of histidine (1 mM)"	"source_name_ch1.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"