GSE26340-GSM646823-GPL11366-PMID:.tsv 1.35 KB
"genotype/variation: wild type M.HpyAVIB"	"characteristics_ch1.1"
"Data extraction from Images was done using Feature Extraction software v 9.5.1 of Agilent. Feature extracted raw data was analyzed using GeneSpring GX V 7.3.1 software from Agilent. Normalization of the data was done in GeneSpring GX using the recommended one color Per Chip and Per Gene Data Transformation: Set measurements less than 0.01 to 0.01, Per Chip: Normalize to 50th percentile, Per Gene: Normalize to Specific Samples. Significant genes up and down regulated showing one fold and above among the samples was identified."	"data_processing.1"
"Bacterial RNA was stabilized in vivo, by using RNA protect Bacteria Reagent (Qiagen). Total RNA was isolated by using RNeasy Kits for RNA purification (Qiagen) as per the manufacturer’s protocol."	"extract_protocol_ch1.1"
"Cells were grown to an OD of 0.4 and then 0.5 mM IPTG was added to induce the protein expression"	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"wild type"	"source_name_ch1.1"
"E. coli BL21(DE3) cells expressing wild-type M.HpyAVIB"	"title.1"
"genotype/variation: wild type M.HpyAVIB"	"characteristics_ch1.1"
"Cells were grown to an OD of 0.4 and then 0.5 mM IPTG was added to induce the protein expression"	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"wild type"	"source_name_ch1.1"