"strain: E.coli O157:H7 EDL 932"	"characteristics_ch1.1"
"strain: E.coli O157:H7 EDL 932"	"characteristics_ch2.1"
"Data analysis was carried out using the Agilent GeneSpring GX. Robust Multi-array Average (RMA) normalization performed using of signal intensity spot data which obtained by scanning process and it is indicated as a scatter plot data. Distribution of all genes which obtained result of the experiment is indicated as a histogram. Accuracy of the results of the experiment was determined through comparison of the histogram with the scatter plot data."	"data_processing.1"
"50µl of total RNA of the cells affected by the Chrysanthemum herba methyl chloride fraction was isolated. The RNA extraction procedure was carried out using the RNeasy mini kit (Qiagen, Inc.) according to the manufacturer’s instructions. Briefly, 1ml of bacterial culture was added to 2ml of RNA protect bacteria reagent (Qiagen, Inc.). Centrifugation (5000 g for 10 minutes) of the mixture was performed to precipitate the cells. The harvested cells were incubated in TE buffer with 1 mg/ml lysozyme (Fisher Scientific). Total RNA was eluted in 50ml of RNase free water (Ambion Inc.) using the NanoDrop Spectrophotometer (NanoDrop Technologies, Inc.). RNA quality was examined using the RNA 6000 Nano Labchip with an Agilent 2100 Bioanalyzer (Agilent Technologies)."	"extract_protocol_ch1.1"
"50µl of total RNA of the cells affected by the Chrysanthemum herba methyl chloride fraction was isolated. The RNA extraction procedure was carried out using the RNeasy mini kit (Qiagen, Inc.) according to the manufacturer’s instructions. Briefly, 1ml of bacterial culture was added to 2ml of RNA protect bacteria reagent (Qiagen, Inc.). Centrifugation (5000 g for 10 minutes) of the mixture was performed to precipitate the cells. The harvested cells were incubated in TE buffer with 1 mg/ml lysozyme (Fisher Scientific). Total RNA was eluted in 50ml of RNase free water (Ambion Inc.) using the NanoDrop Spectrophotometer (NanoDrop Technologies, Inc.). RNA quality was examined using the RNA 6000 Nano Labchip with an Agilent 2100 Bioanalyzer (Agilent Technologies)."	"extract_protocol_ch2.1"
"The bacterial cells were grown at 37℃ for 17 hours on Luria-Bertani (LB) agar plate. An isolated colony was picked and inoculated into 100ml of sterilized LB broth (10g of tryptone, 5g of yeast extract and 10g of sodium chloride per liter) and incubated overnight for 17 hours at 37℃ with shaking at 250 rpm. A 1:100 dilution of the culture was performed using pre-warmed LB broth. The diluted culture was incubated at 37℃ with shaking at 250rpm until a final optical density (OD600) of 0.8 (early logarithmic phase) was attained. A further 1:10 dilution was performed using LB broth and incubated at 37℃ with shaking at 250 rpm."	"growth_protocol_ch1.1"
"The bacterial cells were grown at 37℃ for 17 hours on Luria-Bertani (LB) agar plate. An isolated colony was picked and inoculated into 100ml of sterilized LB broth (10g of tryptone, 5g of yeast extract and 10g of sodium chloride per liter) and incubated overnight for 17 hours at 37℃ with shaking at 250 rpm. A 1:100 dilution of the culture was performed using pre-warmed LB broth. The diluted culture was incubated at 37℃ with shaking at 250rpm until a final optical density (OD600) of 0.8 (early logarithmic phase) was attained. A further 1:10 dilution was performed using LB broth and incubated at 37℃ with shaking at 250 rpm."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"EDL932 exposure to CL MC fraction"	"source_name_ch1.1"
"EDL932 unexposed"	"source_name_ch2.1"
"O157_CL MC fraction vs control"	"title.1"
"200µl of prepared E.coli O157 cells within LB broth is spreaded to LB agar plate, and sterilized 6mm filtered paper disk was placed on the plate tightly, where E.coli O157 is spreaded. Each filtered fractions are injected to each paper disk and Distilled water and Ampicillin were used as negative- and positive control each and then cultured at 37℃ for 24 hours. Finally the diameter of the cleared zone arisen around the paper disk was measured."	"treatment_protocol_ch1.1"
"200µl of prepared E.coli O157 cells within LB broth is spreaded to LB agar plate, and sterilized 6mm filtered paper disk was placed on the plate tightly, where E.coli O157 is spreaded. Each filtered fractions are injected to each paper disk and Distilled water and Ampicillin were used as negative- and positive control each and then cultured at 37℃ for 24 hours. Finally the diameter of the cleared zone arisen around the paper disk was measured."	"treatment_protocol_ch2.1"
"strain: E.coli O157:H7 EDL 932"	"characteristics_ch1.1"
"strain: E.coli O157:H7 EDL 932"	"characteristics_ch2.1"
"The bacterial cells were grown at 37℃ for 17 hours on Luria-Bertani (LB) agar plate. An isolated colony was picked and inoculated into 100ml of sterilized LB broth (10g of tryptone, 5g of yeast extract and 10g of sodium chloride per liter) and incubated overnight for 17 hours at 37℃ with shaking at 250 rpm. A 1:100 dilution of the culture was performed using pre-warmed LB broth. The diluted culture was incubated at 37℃ with shaking at 250rpm until a final optical density (OD600) of 0.8 (early logarithmic phase) was attained. A further 1:10 dilution was performed using LB broth and incubated at 37℃ with shaking at 250 rpm."	"growth_protocol_ch1.1"
"The bacterial cells were grown at 37℃ for 17 hours on Luria-Bertani (LB) agar plate. An isolated colony was picked and inoculated into 100ml of sterilized LB broth (10g of tryptone, 5g of yeast extract and 10g of sodium chloride per liter) and incubated overnight for 17 hours at 37℃ with shaking at 250 rpm. A 1:100 dilution of the culture was performed using pre-warmed LB broth. The diluted culture was incubated at 37℃ with shaking at 250rpm until a final optical density (OD600) of 0.8 (early logarithmic phase) was attained. A further 1:10 dilution was performed using LB broth and incubated at 37℃ with shaking at 250 rpm."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"EDL932 exposure to CL MC fraction"	"source_name_ch1.1"
"EDL932 unexposed"	"source_name_ch2.1"
"200µl of prepared E.coli O157 cells within LB broth is spreaded to LB agar plate, and sterilized 6mm filtered paper disk was placed on the plate tightly, where E.coli O157 is spreaded. Each filtered fractions are injected to each paper disk and Distilled water and Ampicillin were used as negative- and positive control each and then cultured at 37℃ for 24 hours. Finally the diameter of the cleared zone arisen around the paper disk was measured."	"treatment_protocol_ch1.1"
"200µl of prepared E.coli O157 cells within LB broth is spreaded to LB agar plate, and sterilized 6mm filtered paper disk was placed on the plate tightly, where E.coli O157 is spreaded. Each filtered fractions are injected to each paper disk and Distilled water and Ampicillin were used as negative- and positive control each and then cultured at 37℃ for 24 hours. Finally the diameter of the cleared zone arisen around the paper disk was measured."	"treatment_protocol_ch2.1"