GSE25745-GSM632645-GPL3154-PMID:21169441.tsv
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"strain: CFT073" "characteristics_ch1.1"
"growth condition: LB broth supplemented with cranberry PACs (100 µg/mL)" "characteristics_ch1.2"
"The data were analyzed with FlexArray 1.4.1 software program (http://genomequebec.mcgill.ca/FlexArray/). The data were normalized using the Robust Multi-Array Average algorithm (RMA). Comparison between the two data sets was done using the Randomized Variance Model (RVM) with a cut-off of a fold change of 2 (or -2) and a P-value of 0.05." "data_processing.1"
"RNA extraction was performed according to the manufacturer's (Qiagen) instructions." "extract_protocol_ch1.1"
"Cultures of E. coli CFT073 were maintained at –80ºC in Luria-Bertani broth with 15% glycerol. Cultures were streaked onto LB agar plates and incubated (37°C, 24 h). A single colony was inoculated into 150 mL of LB broth (in a 500 mL baffled flask). Three inoculated flasks contained LB broth alone (controls), and three inoculated flasks were supplemented with cranberry derived PAC extract (100 µg/mL).Bacteria were incubated at 37°C, 5 h, 200 rpm to mid-log growth phase." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"CFT073 in LB+PACs (100 µg/mL), after 5 hours of growth." "source_name_ch1.1"
"CFT073_LB+PACs_rep2" "title.1"
"One volume of bacterial culture was added to two volumes of RNAprotect Bacteria Reagent (Qiagen) and RNA was extracted using RNeasy mini kit (Qiagen). All samples were on-column treated with DNase I." "treatment_protocol_ch1.1"
"strain: CFT073" "characteristics_ch1.1"
"growth condition: LB broth supplemented with cranberry PACs (100 µg/mL)" "characteristics_ch1.2"
"Cultures of E. coli CFT073 were maintained at –80ºC in Luria-Bertani broth with 15% glycerol. Cultures were streaked onto LB agar plates and incubated (37°C, 24 h). A single colony was inoculated into 150 mL of LB broth (in a 500 mL baffled flask). Three inoculated flasks contained LB broth alone (controls), and three inoculated flasks were supplemented with cranberry derived PAC extract (100 µg/mL).Bacteria were incubated at 37°C, 5 h, 200 rpm to mid-log growth phase." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"CFT073 in LB+PACs (100 µg/mL), after 5 hours of growth." "source_name_ch1.1"
"One volume of bacterial culture was added to two volumes of RNAprotect Bacteria Reagent (Qiagen) and RNA was extracted using RNeasy mini kit (Qiagen). All samples were on-column treated with DNase I." "treatment_protocol_ch1.1"