GSE25601-GSM629015-GPL10979-PMID:.tsv 2.04 KB
"strain: BCE049, DS3"	"characteristics_ch1.1"
"strain: B7A"	"characteristics_ch2.1"
"1. Use Agilent software Feature Extraction to analyze spotted arrays."	"data_processing.1"
"2. Use TM4 suite software ExpressConverter to modify Agilent Feature Extraction file into *.mev. "	"data_processing.2"
"3. Calculate the value of log2 ratio (Reference/Query) from the normalized .mev file:"	"data_processing.3"
"    3a). If both channels (QUERY_MEDIAN_INTENSITY and REF_MEDIAN_INTENSITY) are zero, assign the value \"null\"."	"data_processing.4"
"    3b). If one channel is zero and the other is not zero, substitute the zero with one (1) and then, assign the log2 ratio of QUERY_MEDIAN_INTENSITY/REF_MEDIAN_INTENSITY to VALUE"	"data_processing.5"
"    3c). If neither channel is zero, assign the log2 ratio of QUERY_MEDIAN_INTENSITY/REF_MEDIAN_INTENSITY to VALUE."	"data_processing.6"
"Genomic DNA was isolated by lysozyme and proteinase K treatment."	"extract_protocol_ch1.1"
"Genomic DNA was isolated by lysozyme and proteinase K treatment."	"extract_protocol_ch2.1"
"cells were grown in LB overnight at 37 degrees celcius."	"growth_protocol_ch1.1"
"cells were grown in LB overnight at 37 degrees celcius."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Genomic DNA purified from wild type bacteria"	"source_name_ch1.1"
"Genomic DNA purified from wild type bacteria"	"source_name_ch2.1"
"252978410032_B7A_CY5_BCE049_DS3_CY3.mev.refIsIB.out"	"title.1"
"None"	"treatment_protocol_ch1.1"
"None"	"treatment_protocol_ch2.1"
"strain: BCE049, DS3"	"characteristics_ch1.1"
"strain: B7A"	"characteristics_ch2.1"
"cells were grown in LB overnight at 37 degrees celcius."	"growth_protocol_ch1.1"
"cells were grown in LB overnight at 37 degrees celcius."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Genomic DNA purified from wild type bacteria"	"source_name_ch1.1"
"Genomic DNA purified from wild type bacteria"	"source_name_ch2.1"
"None"	"treatment_protocol_ch1.1"
"None"	"treatment_protocol_ch2.1"