GSE25601-GSM628779-GPL10979-PMID:.tsv
2.02 KB
"strain: O63:nm" "characteristics_ch1.1"
"strain: B7A" "characteristics_ch2.1"
"1. Use Agilent software Feature Extraction to analyze spotted arrays." "data_processing.1"
"2. Use TM4 suite software ExpressConverter to modify Agilent Feature Extraction file into *.mev. " "data_processing.2"
"3. Calculate the value of log2 ratio (Reference/Query) from the normalized .mev file:" "data_processing.3"
" 3a). If both channels (QUERY_MEDIAN_INTENSITY and REF_MEDIAN_INTENSITY) are zero, assign the value \"null\"." "data_processing.4"
" 3b). If one channel is zero and the other is not zero, substitute the zero with one (1) and then, assign the log2 ratio of QUERY_MEDIAN_INTENSITY/REF_MEDIAN_INTENSITY to VALUE" "data_processing.5"
" 3c). If neither channel is zero, assign the log2 ratio of QUERY_MEDIAN_INTENSITY/REF_MEDIAN_INTENSITY to VALUE." "data_processing.6"
"Genomic DNA was isolated by lysozyme and proteinase K treatment." "extract_protocol_ch1.1"
"Genomic DNA was isolated by lysozyme and proteinase K treatment." "extract_protocol_ch2.1"
"cells were grown in LB overnight at 37 degrees celcius." "growth_protocol_ch1.1"
"cells were grown in LB overnight at 37 degrees celcius." "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Genomic DNA purified from wild type bacteria" "source_name_ch1.1"
"Genomic DNA purified from wild type bacteria" "source_name_ch2.1"
"252978410072_B7A_CY5_O63_nm_CY3.mev.refIsIB.out" "title.1"
"None" "treatment_protocol_ch1.1"
"None" "treatment_protocol_ch2.1"
"strain: O63:nm" "characteristics_ch1.1"
"strain: B7A" "characteristics_ch2.1"
"cells were grown in LB overnight at 37 degrees celcius." "growth_protocol_ch1.1"
"cells were grown in LB overnight at 37 degrees celcius." "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Genomic DNA purified from wild type bacteria" "source_name_ch1.1"
"Genomic DNA purified from wild type bacteria" "source_name_ch2.1"
"None" "treatment_protocol_ch1.1"
"None" "treatment_protocol_ch2.1"