GSE24353-GSM674761-GPL10926-PMID:21503758.tsv
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"isolation source: Mixed culture" "characteristics_ch1.1"
"Images were quantified using Agilent Feature Extraction Software (10.5.1.1) and obtained background subtracted and spatially detrended Processed Signal intensities." "data_processing.1"
"The genomic DNA from pure and mixed bacterial cultures was isolated using the Wizard genomic DNA isolation kit (Promega corporation, Madison, USA) as per manufacturer's instructions. The concentration of DNA was analyzed by Nanodrop spectrophotometer (Nanodrop Technologies Inc, Rockland, USA), and quality was determined by analysis on DNA 12000 kit (Caliper Sciences, USA) using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA)." "extract_protocol_ch1.1"
"The Sphingomonas sp. strain NM-05 used in the study is a known degrader of γ-hexachlorocyclohexane (γ-HCH) and has been isolated from the vicinity of an industry India Pesticide Limited, Chinhat industrial area, Lucknow which is involved in manufacturing chlorinated pesticides for last 25 years. The strain was harvested in mineral salt medium with 0.34mM γ-HCH as sole source of carbon and energy. The strain Bordetella sp. strain IITR-02 used in this study has been isolated from the vicinity of the industry India Pesticide Limited, Chinhat, Lucknow and is found to be a degrader of 1,2-, 1,4-dichloro and 1,2,3- and 1,2,4-trichlorobenzene. The strain was harvested in mineral salt medium with 3.2mM of 1,2,4-trichlorobenzene as sole source of carbon and energy. The bacterium Rhodococcus sp. strain RHA1 which is a known degrader of polychlorinated biphenyls, substituted phenols, benzoates and phthalates was grown on 0.2% biphenyl and 20mM of benzoate as carbon sources. The strain Escherichia coli DH5α and Escherichia coli BL21 were grown in Luria-Bertani medium and strain Escherichia coli K12 was grown in nutrient broth to an OD560nm of 0.4 at 37 degree centigrade with continuous shaking." "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"Sphingomonas sp. NM05" "organism_ch1.2"
"Escherichia coli BL21" "organism_ch1.3"
"Escherichia coli DH5[alpha]" "organism_ch1.4"
"Genomic DNA of mixed culture containing only non-degraders in equal ratio" "source_name_ch1.1"
"MC_Val_G5" "title.1"
"isolation source: Mixed culture" "characteristics_ch1.1"
"The Sphingomonas sp. strain NM-05 used in the study is a known degrader of γ-hexachlorocyclohexane (γ-HCH) and has been isolated from the vicinity of an industry India Pesticide Limited, Chinhat industrial area, Lucknow which is involved in manufacturing chlorinated pesticides for last 25 years. The strain was harvested in mineral salt medium with 0.34mM γ-HCH as sole source of carbon and energy. The strain Bordetella sp. strain IITR-02 used in this study has been isolated from the vicinity of the industry India Pesticide Limited, Chinhat, Lucknow and is found to be a degrader of 1,2-, 1,4-dichloro and 1,2,3- and 1,2,4-trichlorobenzene. The strain was harvested in mineral salt medium with 3.2mM of 1,2,4-trichlorobenzene as sole source of carbon and energy. The bacterium Rhodococcus sp. strain RHA1 which is a known degrader of polychlorinated biphenyls, substituted phenols, benzoates and phthalates was grown on 0.2% biphenyl and 20mM of benzoate as carbon sources. The strain Escherichia coli DH5α and Escherichia coli BL21 were grown in Luria-Bertani medium and strain Escherichia coli K12 was grown in nutrient broth to an OD560nm of 0.4 at 37 degree centigrade with continuous shaking." "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"Sphingomonas sp. NM05" "organism_ch1.2"
"Escherichia coli BL21" "organism_ch1.3"
"Escherichia coli DH5[alpha]" "organism_ch1.4"
"Genomic DNA of mixed culture containing only non-degraders in equal ratio" "source_name_ch1.1"