"strain: 86-24"	"characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting"	"data_processing.1"
"E. coli O157:H7 86-24 was inoculated in 25 ml of LB in 250 ml shake flasks with overnight cultures that were diluted 1:100. Cells were shaken at 250 rpm and 37°C for an absorbance of 4.0 at 600 nm.  Cells were immediately chilled with dry ice and 95% ethanol (to prevent RNA degradation) for 30 sec before centrifugation at 13,000 g for 2 min; cell pellets were frozen immediately with dry ice and stored -80°C.  RNA was isolated using Qiagen RNeasy mini Kit (Cat# 74104)."	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Channel 1"	"source_name_ch1.1"
"E. coli O157:H7 86-24 in LB at 37oC at OD 4.0"	"title.1"
"strain: 86-24"	"characteristics_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Channel 1"	"source_name_ch1.1"