GSE17853-GSM445915-GPL3154-PMID:.tsv
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"strain: BW25113 hha hns / pCA24N-hnsK57N" "characteristics_ch1.1"
"time: 7 hours" "characteristics_ch1.2"
"medium: LB" "characteristics_ch1.3"
"cell type: biofilm" "characteristics_ch1.4"
"growth protocol: biofilm grown on glass wool, 37C" "characteristics_ch1.5"
"MAS 5.0 Expression Analysis Default Setting" "data_processing.1"
"The overnight culture (2.5 ml) was used to inoculate 250 ml of fresh LB medium with 10 g of submerged glass wool (Corning Glass Works, Corning, NY) for forming biofilm. After incubation for 7 h at 37°C with shaking (250 rpm), the glass wool was carefully and quickly removed and rinsed with 100 ml of sterile 0.85% NaCl solution at 0°C. Biofilm cells were removed by sonicating the glass wool in 200 ml of sterile 0.85% NaCl solution at 0°C. After breaking the cells with a bead beater, and the total RNA was isolated with Qiagen RNeasy mini Kit (Cat# 74104)." "extract_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"pCA24N-hnsK57N biofilm" "source_name_ch1.1"
"pCA24N-hnsK57N biofilm" "title.1"
"strain: BW25113 hha hns / pCA24N-hnsK57N" "characteristics_ch1.1"
"time: 7 hours" "characteristics_ch1.2"
"medium: LB" "characteristics_ch1.3"
"cell type: biofilm" "characteristics_ch1.4"
"growth protocol: biofilm grown on glass wool, 37C" "characteristics_ch1.5"
"Escherichia coli K-12" "organism_ch1.1"
"pCA24N-hnsK57N biofilm" "source_name_ch1.1"