GSE17517-GSM436578-GPL8960-PMID:19684169.tsv 2.52 KB
"year: 2000"	"characteristics_ch1.1"
"contamination status: hydrocarbon contaminated"	"characteristics_ch1.2"
"sample: 1A"	"characteristics_ch1.3"
"phage: Lambda"	"characteristics_ch2.1"
"The intensity of each spot was measured with ScanArray Express using the histogram quantitation method; the percentile ranges for histograms were 80% for low signal range, 95% for high signal range, 5% for low background range and 20% for high background range. The median intensity minus the median background intensity was used for further calculations performed in Microsoft Excel. The background measurement refers to the local spot background intensity. Prior to normalization, all spots with a Signal-to-Noise ratio (SNR) lower than 3 were removed. The SNR was calculated using the following formula: (Median Signal – Median Background)/Standard Deviation of Background. Normalization between each array was performed using an internal labeling control (IC) consisting of a 290 bp luxA fragment. This fragment was added to each labeling reaction at a ratio of 0.05% of the DNA to be labeled. The intensity of each Cy5-sample gene spot  was corrected using the average intensity of all Cy3-lambda signals. Corrected intensity values were averaged over triplicate spots to give a single value per gene. The intensity of the genes for which hybridization of the probes could not be visually detected in image analysis was set to zero. Relative intensity values were then calculated by dividing the intensity of a gene by the sum of the intensity for all genes (excluding controls). This “relative abundance” represents the fraction of the total intensity that is due to a particular gene and this value was subsequently used in statistical analyses."	"data_processing.1"
"Soil DNA was extracted from a 0.5g soil sub-sample using the MoBio DNA Power Soil kit (MoBio Laboratories, Carlsbad, CA)."	"extract_protocol_ch1.1"
"Soil DNA was extracted from a 0.5g soil sub-sample using the MoBio DNA Power Soil kit (MoBio Laboratories, Carlsbad, CA)."	"extract_protocol_ch2.1"
"Bacteria"	"organism_ch1.1"
"Archaea"	"organism_ch1.2"
"Escherichia coli"	"organism_ch2.1"
"Eureka"	"source_name_ch1.1"
"Lambda phage"	"source_name_ch2.1"
"1A T0"	"title.1"
"year: 2000"	"characteristics_ch1.1"
"contamination status: hydrocarbon contaminated"	"characteristics_ch1.2"
"sample: 1A"	"characteristics_ch1.3"
"phage: Lambda"	"characteristics_ch2.1"
"Bacteria"	"organism_ch1.1"
"Archaea"	"organism_ch1.2"
"Escherichia coli"	"organism_ch2.1"
"Eureka"	"source_name_ch1.1"
"Lambda phage"	"source_name_ch2.1"